ArticleInfection and immunity2012
Legionella pneumophila pathogenesis in the Galleria mellonella infection model.
Article in Infection and immunity, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 111 citations in OpenAlex.
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- FleQ-Dependent regulation of the ribonucleotide reductase repressor NrdR in Pseudomonas aeruginosa during biofilm growth and infection.Scientific reports · 2025Article
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- Investigating Secretion Systems and Effectors on Galleria mellonella.Methods in molecular biology (Clifton, N.J.) · 2024Article
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- The role of type VI secretion system genes in antibiotic resistance and virulence inFrontiers in cellular and infection microbiology · 2024Article
- Phagocytosis-associated genes in Acanthamoeba castellanii feeding on Escherichia coli.Parasites, hosts and diseases · 2023Article
- Identifying the function of genes involved in excreted vesicle formation in Acanthamoeba castellanii containing Legionella pneumophila.Parasites & vectors · 2023Article
- Hiding in the yolk: A unique feature of Legionella pneumophila infection of zebrafish.PLoS pathogens · 2023Article
- The VirtuousAntibiotics (Basel, Switzerland) · 2023Review
- Light-activated molecular machines are fast-acting broad-spectrum antibacterials that target the membrane.Science advances · 2022Article
- Third generation cephalosporin-resistant Klebsiella pneumoniae thriving in patients and in wastewater: what do they have in common?BMC genomics · 2022Article
- The utilization of Blaptica dubia cockroaches as an in vivo model to test antibiotic efficacy.Scientific reports · 2021Article
- The Impact of Graphite Oxide Nanocomposites on the Antibacterial Activity of Serum.International journal of molecular sciences · 2021Article
- Role of CgTpo4 in Polyamine and Antimicrobial Peptide Resistance: Determining Virulence inInternational journal of molecular sciences · 2021Article
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- Functional Analysis of a Fibronectin Binding Protein of Streptococcus parasanguinis FW213.Current microbiology · 2020Article
- The anti-apoptotic Coxiella burnetii effector protein AnkG is a strain specific virulence factor.Scientific reports · 2020Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Legionella pneumophila is a facultative intracellular human pathogen and the etiological agent of severe pneumonia known as Legionnaires' disease. Its virulence depends on protein secretion systems, in particular, the Dot/Icm type IV secretion system (T4SS), which is essential to establish a replication-permissive vacuole in macrophages. The analysis of the role of these systems and their substrates for pathogenesis requires easy-to-use models which approximate human infection. We examined the effectiveness of the larvae of the wax moth Galleria mellonella as a new model for L. pneumophila infection. We found that the L. pneumophila strains 130b, Paris, and JR32 caused mortality of the G. mellonella larvae that was strain, infectious dose, growth phase, and T4SS dependent. Wild-type L. pneumophila persisted and replicated within the larvae, whereas T4SS mutants were rapidly cleared. L. pneumophila strain Lp02, which is attenuated in the absence of thymidine but has a functional T4SS, resisted clearance in G. mellonella up to 18 h postinfection without inducing mortality. Immunofluorescence and transmission electron microscopy revealed that L. pneumophila resided within insect hemocytes in a vacuole that ultrastructurally resembled the Legionella-containing vacuole (LCV) observed in macrophages. The vacuole was decorated with the T4SS effector and LCV marker SidC. Infection caused severe damage to the insect organs and triggered immune responses, including activation of the phenoloxidase cascade leading to melanization, nodule formation, and upregulation of antimicrobial peptides. Taken together, these results suggest that G. mellonella provides an effective model to investigate the interaction between L. pneumophila and the host.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.