ArticleInfection and immunity2012
Pneumococcal interactions with epithelial cells are crucial for optimal biofilm formation and colonization in vitro and in vivo.
Article in Infection and immunity, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 104 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
104 citing papers in PubMed, 152 citations in OpenAlex.
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- Analysis of pathogen distribution and risk factors for extended antibiotic course in children with microbiological-based protracted bacterial bronchitis in southwest China.Frontiers in pediatrics · 2026Article
- Biofilm formation during pneumococcal carriage imprints naturally acquired humoral immunity.bioRxiv : the preprint server for biology · 2025Article
- Label-free microscopy enables high-throughput identification of genes controlling biofilm development.bioRxiv : the preprint server for biology · 2025Article
- Biofilm characterisation of Mycoplasma bovis co-cultured with Trueperella pyogenes.Veterinary research · 2025Article
- Point mutations in functionally diverse genes are associated with increased natural DNA transformation in multidrug resistant Streptococcus pneumoniae.Nucleic acids research · 2025Article
- Ultrastructural, metabolic and genetic characteristics of determinants facilitating the acquisition of macrolide resistance by Streptococcus pneumoniae.Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy · 2024Article
- Biological puzzles solved by usingJournal of bacteriology · 2024Review
- Time-resolved RNA-seq analysis to unravel theMicrobiology spectrum · 2024Article
- Role of the polyamine transporter PotABCD during biofilm formation by Streptococcus pneumoniae.PloS one · 2024Article
- Biofilm-dispersed pneumococci induce elevated leukocyte and platelet activation.Frontiers in cellular and infection microbiology · 2024Article
- The choline-binding proteins PspA, PspC, and LytA ofInfection and immunity · 2023Article
- Potential of Desert Medicinal Plants for Combating Resistant Biofilms in Urinary Tract Infections.Applied biochemistry and biotechnology · 2023Review
- Airway proteolytic control of pneumococcal competence.PLoS pathogens · 2023Article
- Influence of glycan structure on the colonization ofProceedings of the National Academy of Sciences of the United States of America · 2023Article
- The quorum sensing com system regulates pneumococcal colonisation and invasive disease in a pseudo-stratified airway tissue model.Microbiological research · 2023Article
- Measuring Niche-Associated Metabolic Activity in Planktonic and Biofilm Bacteria.Methods in molecular biology (Clifton, N.J.) · 2023Article
44 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The human nasopharynx is the main reservoir for Streptococcus pneumoniae (the pneumococcus) and the source for both horizontal spread and transition to infection. Some clinical evidence indicates that nasopharyngeal carriage is harder to eradicate with antibiotics than is pneumococcal invasive disease, which may suggest that colonizing pneumococci exist in biofilm communities that are more resistant to antibiotics. While pneumococcal biofilms have been observed during symptomatic infection, their role in colonization and the role of host factors in this process have been less studied. Here, we show for the first time that pneumococci form highly structured biofilm communities during colonization of the murine nasopharynx that display increased antibiotic resistance. Furthermore, pneumococcal biofilms grown on respiratory epithelial cells exhibited phenotypes similar to those observed during colonization in vivo, whereas abiotic surfaces produced less ordered and more antibiotic-sensitive biofilms. The importance of bacterial-epithelial cell interactions during biofilm formation was shown using both clinical strains with variable colonization efficacies and pneumococcal mutants with impaired colonization characteristics in vivo. In both cases, the ability of strains to form biofilms on epithelial cells directly correlated with their ability to colonize the nasopharynx in vivo, with colonization-deficient strains forming less structured and more antibiotic-sensitive biofilms on epithelial cells, an association that was lost when grown on abiotic surfaces. Thus, these studies emphasize the importance of host-bacterial interactions in pneumococcal biofilm formation and provide the first experimental data to explain the high resistance of pneumococcal colonization to eradication by antibiotics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.