ArticleThe Journal of membrane biology2012
In vivo molecular imaging and histological analysis of changes induced by electric pulses used for plasmid DNA electrotransfer to the skin: a study in a dorsal window chamber in mice.
Article in The Journal of membrane biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 45 citations in OpenAlex.
- In silico study about the influence of electroporation parameters on the cellular internalization, spatial uniformity, and cytotoxic effects of chemotherapeutic drugs using the Method of Fundamental Solutions.Medical & biological engineering & computing · 2024Article
- Influence of electric field, blood velocity, and pharmacokinetics on electrochemotherapy efficiency.Biophysical journal · 2023Article
- Transdermal Drug Delivery in the Pig Skin.Pharmaceutics · 2021Article
- High Power Electromagnetic Waves Exposure of Healthy and Tumor Bearing Mice: Assessment of Effects on Mice Growth, Behavior, Tumor Growth, and Vessel Permeabilization.International journal of molecular sciences · 2021Article
- Electroporation-induced changes in tumor vasculature and microenvironment can promote the delivery and increase the efficacy of sorafenib nanoparticles.Bioelectrochemistry (Amsterdam, Netherlands) · 2019Article
- Effect of calcium electroporation on tumour vasculature.Scientific reports · 2018Article
- Mathematical model of tumor volume dynamics in mice treated with electrochemotherapy.Medical & biological engineering & computing · 2017Article
- Electroporation as a vaccine delivery system and a natural adjuvant to intradermal administration of plasmid DNA in macaques.Scientific reports · 2017Article
- Electrotransfer parameters as a tool for controlled and targeted gene expression in skin.Molecular therapy. Nucleic acids · 2016Article
- Improved Specificity of Gene Electrotransfer to Skin Using pDNA Under the Control of Collagen Tissue-Specific Promoter.The Journal of membrane biology · 2015Article
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- Biological properties of melanoma and endothelial cells after plasmid AMEP gene electrotransfer depend on integrin quantity on cells.The Journal of membrane biology · 2013Article
- Article
- Potentiation of electrochemotherapy by intramuscular IL-12 gene electrotransfer in murine sarcoma and carcinoma with different immunogenicity.Radiology and oncology · 2012Article
- Differential cellular effects of electroporation and electrochemotherapy in monolayers of human microvascular endothelial cells.PloS one · 2012Article
- Reversible Electroporation-Mediated Liposomal Doxorubicin Delivery to Tumors Can Be Monitored WithMolecular imagingArticle
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Electropermeabilization/electroporation (EP) is a physical method that by application of electric pulses to cells increases cell membrane permeability and enables the introduction of molecules into the cells. One of the uses of EP in vivo is plasmid DNA electrotransfer to the skin for DNA vaccination. EP of tissues induces reduction of blood flow and, in combination with plasmid DNA, induction of an immune response. One of the EP protocols for plasmid DNA electrotransfer to the skin is a combination of high-voltage (HV) and low-voltage (LV) pulses. However, the effects of this pulse combination on skin-vessel blood flow are not known. Therefore, using intravital microscopy in a dorsal window chamber in mice and fluorescently labeled dextrans, the effects of one HV and eight LV pulses on skin vasculature were investigated. In addition, a detailed histological analysis was performed. Image analysis of fluorescence intensity changes demonstrated that EP induces a transient constriction and increased permeability of blood vessels as well as a "vascular lock." Histological analysis revealed rounding up of endothelial cells and stacking up of erythrocytes at 1 h after EP. In addition, extravasation of erythrocytes and leukocyte infiltration accompanied by edema were determined up to 24 h after EP. In conclusion, our results show that blood flow modifying effects of EP in skin contribute to the infiltration of immune cells in the exposed area. When combined with plasmid DNA for vaccination, this could enable the initial and prolonged contact of immune cells with encoded therapeutic proteins.
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