ArticleHistochemistry and cell biology2012
An apical actin-rich domain drives the establishment of cell polarity during cell adhesion.
Article in Histochemistry and cell biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- PUS7 pseudouridylation of 7SK orchestrates human somatic cell reprogramming into iPS cells.Nature chemical biology · 2026Article
- CD93 in Health and Disease: Bridging Physiological Functions and Clinical Applications.International journal of molecular sciences · 2025Review
- Semi-synthetic fibrous fibrin composites promote 3D microvascular assembly, survival, and host integration of endothelial cells without mesenchymal cell support.Bioactive materials · 2025Article
- Shroom3 facilitates optic fissure closure via tissue alignment and reestablishment of apical-basal polarity during epithelial fusion.Developmental biology · 2025Review
- Role of CD93 in Health and Disease.Cells · 2023Review
- Article
- Actin cytoskeleton in angiogenesis.Biology open · 2022Review
- Hepatocyte growth factor-regulated tyrosine kinase substrate is essential for endothelial cell polarity and cerebrovascular stability.Cardiovascular research · 2021Article
- The Relative Densities of Cytoplasm and Nuclear Compartments Are Robust against Strong Perturbation.Biophysical journal · 2020Article
- The Binding of CD93 to Multimerin-2 Promotes Choroidal Neovascularization.Investigative ophthalmology & visual science · 2020Article
- Histochemistry and Cell Biology: 61 years and not tired at all.Histochemistry and cell biology · 2019Article
- The small GTPase Rab5c is a key regulator of trafficking of the CD93/Multimerin-2/β1 integrin complex in endothelial cell adhesion and migration.Cell communication and signaling : CCS · 2019Article
- CD93 and dystroglycan cooperation in human endothelial cell adhesion and migration adhesion and migration.Oncotarget · 2016Article
- The characterization of a novel monoclonal antibody against CD93 unveils a new antiangiogenic target.Oncotarget · 2014Article
- The Histochemistry and Cell Biology compendium: a review of 2012.Histochemistry and cell biology · 2013Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
One of the most important questions in cell biology concerns how cells reorganize after sensing polarity cues. In the present study, we describe the formation of an actin-rich domain on the apical surface of human primary endothelial cells adhering to the substrate and investigate its role in cell polarity. We used confocal immunofluorescence procedures to follow the redistribution of proteins required for endothelial cell polarity during spreading initiation. Activated Moesin, vascular endothelial cadherin and partitioning defective 3 were found to be localized in the apical domain, whereas podocalyxin and caveolin-1 were distributed along the microtubule cytoskeleton axis, oriented from the centrosome to the cortical actin-rich domain. Moreover, activated signaling molecules were localized in the core of the apical domain in tight association with filamentous actin. During cell attachment, loss of the apical domain by Moesin silencing or drug disruption of the actin cytoskeleton caused irregular cell spreading and mislocalization of polarity markers. In conclusion, our results suggest that the apical domain that forms during the spreading process is a structural organizer of cell polarity by regulating trafficking and activation of signaling proteins.
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