ArticleMolecules and cells2012
Regulation of actin cytoskeleton by Rap1 binding to RacGEF1.
Article in Molecules and cells, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it, 35 citations in OpenAlex.
- Genetic determinants of skin ageing: a systematic review and meta-analysis of genome-wide association studies and candidate genes.Journal of physiological anthropology · 2025Pooled it
- DNA methylation changes following narrative exposure therapy in a randomized controlled trial with female former child soldiers.Scientific reports · 2021Trial
- RapB Regulates Cell Adhesion and Migration in Dictyostelium, Similar to RapA.Journal of microbiology (Seoul, Korea) · 2024Article
- Proteomics and phosphoproteomics reveal novel proteins involved inFrontiers in chemistry · 2024Article
- Article
- Identification of phenotype-specific networks from paired gene expression-cell shape imaging data.Genome research · 2022Article
- Fucoidan (Marine drugs · 2022Article
- PXF-1 promotes synapse development at the neuromuscular junction inFrontiers in molecular neuroscience · 2022Article
- The miR-133a, TPM4 and TAp63γ Role in Myocyte Differentiation Microfilament Remodelling and Colon Cancer Progression.International journal of molecular sciences · 2021Article
- Reversible function of RapA with the C-terminus of RapC in Dictyostelium.Journal of microbiology (Seoul, Korea) · 2021Article
- Opposite functions of RapA and RapC in cell adhesion and migration inAnimal cells and systems · 2021Article
- Changes in the proteome of sea urchin Paracentrotus lividus coelomocytes in response to LPS injection into the body cavity.PloS one · 2020Article
- Role of the small GTPase Rap1 in signal transduction, cell dynamics and bacterial infection.Small GTPases · 2019Review
- Hepatic Glucagon Signaling Regulates PCSK9 and Low-Density Lipoprotein Cholesterol.Circulation research · 2019Article
- YAP/TAZ Related BioMechano Signal Transduction and Cancer Metastasis.Frontiers in cell and developmental biology · 2019Review
- Selectins and chemokines use shared and distinct signals to activate β2 integrins in neutrophils.Blood advances · 2018Article
- Rap1 Negatively Regulates the Hippo Pathway to Polarize Directional Protrusions in Collective Cell Migration.Cell reports · 2018Article
- The Rap activator Gef26 regulates synaptic growth and neuronal survival via inhibition of BMP signaling.Molecular brain · 2017Article
- Protein kinase A regulates the Ras, Rap1 and TORC2 pathways in response to the chemoattractant cAMP inJournal of cell science · 2017Article
- Comparative Proteomics of Purified Pathogen Vacuoles Correlates Intracellular Replication ofMolecular & cellular proteomics : MCP · 2017Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rap1 is rapidly and transiently activated in response to chemoattractant stimulation and helps establish cell polarity by locally modulating cytoskeletons. Here, we investigated the mechanisms by which Rap1 controls actin cytoskeletal reorganization in Dictyostelium and found that Rap1 interacts with RacGEF1 in vitro and stimulates F-actin polymerization at the sites where Rap1 is activated upon chemoattractant stimulation. Live cell imaging using GFP-coronin, a reporter for F-actin, demonstrates that cells expressing constitutively active Rap1 (Rap1CA) exhibit a high level of F-actin uniformly distributed at the cortex including the posterior and lateral sides of the chemotaxing cell. Examination of the localization of a PH-domain containing PIP3 reporter, PhdA-GFP, and the activation of Akt/Pkb and other Ras proteins in Rap1CA cells reveals that activated Rap1 has no effect on the production of PIP3 or the activation of Akt/Pkb and Ras proteins in response to chemoattractant stimulation. Rac family proteins are crucial regulators in actin cytoskeletal reorganization. In vitro binding assay using truncated RacGEF1 proteins shows that Rap1 interacts with the DH domain of RacGEF1. Taken together, these results suggest that Rap1-mediated F-actin polymerization probably occurs through the Rac signaling pathway by directly binding to RacGEF1.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.