ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2012
MALDI-TOF mass spectrometry and microsatellite markers to evaluate Candida parapsilosis transmission in neonatal intensive care units.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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35 citing papers in PubMed, 72 citations in OpenAlex.
- A Retrospective Molecular Epidemiology Study: Emergence and dissemination of Azole-Resistant Candida parapsilosis Sensu Stricto in Southern Brazil.Mycopathologia · 2026Article
- Azole Resistance in Candida parapsilosis From Patients With Burns in Mexico: A Genomic and Phylogenetic Analysis.Mycoses · 2026Article
- Silent Persistence: Molecular Evidence of Clonal Transmission in Fluconazole-ResistantJournal of fungi (Basel, Switzerland) · 2025Article
- Nine-Year Surveillance ofJournal of fungi (Basel, Switzerland) · 2025Article
- Mass spectrometry-based proteomics as an emerging tool in clinical laboratories.Clinical proteomics · 2023Review
- A pseudo-outbreak ofFrontiers in cellular and infection microbiology · 2023Article
- Genotypic Diversity ofJournal of fungi (Basel, Switzerland) · 2022Article
- Determinants of fluconazole resistance and echinocandin tolerance inEmerging microbes & infections · 2022Article
- A Brazilian Inter-Hospital Candidemia Outbreak Caused by Fluconazole-ResistantJournal of fungi (Basel, Switzerland) · 2022Article
- Environmental Clonal Spread of Azole-ResistantJournal of fungi (Basel, Switzerland) · 2021Article
- Urinary Tract Infections Caused by Fluconazole-ResistantOpen forum infectious diseases · 2020Article
- Evaluation of Microsatellite Typing, ITS Sequencing, AFLP Fingerprinting, MALDI-TOF MS, and Fourier-Transform Infrared Spectroscopy Analysis ofJournal of fungi (Basel, Switzerland) · 2020Article
- Tuberomics: a molecular profiling for the adaption of edible fungi (Tuber magnatum Pico) to different natural environments.BMC genomics · 2020Article
- Molecular Characterization ofFrontiers in microbiology · 2020Article
- Pathogen proteotyping: A rapidly developing application of mass spectrometry to address clinical concerns.Clinical mass spectrometry (Del Mar, Calif.) · 2019Review
- High-resolution fingerprinting of Candida parapsilosis isolates suggests persistence and transmission of infections among neonatal intensive care unit patients in Kuwait.Scientific reports · 2019Article
- Molecular Genotyping of Candida parapsilosis Species Complex.Mycopathologia · 2018Article
- Design and validation of a multiplex PCR protocol for microsatellite typing of Candida parapsilosis sensu stricto isolates.BMC genomics · 2018Article
- Article
- An Azole-ResistantFrontiers in microbiology · 2018Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent studies on outbreaks of Candida showed an increased incidence of bloodstream infections in neonatal intensive care units (NICUs) caused by C. parapsilosis species, highlighting the need for the proper identification and epidemiology of these species. Several systems are available for molecular epidemiological and taxonomic studies of fungal infections: pulsed-field gel electrophoresis (PFGE) represents the gold standard for typing, but is also one of the most lengthy and expensive, while simple sequence repeats (SSRs) is based on polymerase chain reaction (PCR) amplification and is, therefore, faster. Only recently, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been used to identify and type microorganisms involved in nosocomial outbreaks. In our study, 19 strains of C. parapsilosis isolated from the blood cultures of neonates admitted to the University Hospital Federico II were genotyped by the amplification of eight SSR markers and by MALDI-TOF MS. Electrophoretic and spectrometric profile results were compared in order to identify similarities among the isolates and to study microevolutionary changes in the C. parapsilosis population. The discriminatory power and the unweighted pair group method with arithmetic mean (UPGMA) dendrograms generated were compared in order to evaluate the correlation of the groups established by the analysis of the clusters by both methods. Both methods were rapid and effective in highlighting identical strains and studying microevolutionary changes in the population. Our study evidenced that mass spectroscopy is a useful technique not only for the identification but also for monitoring the spread of strains, which is critical to control nosocomial infections.
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