ArticleNucleic acids research2012
Hydrogen bond formation between the naturally modified nucleobase and phosphate backbone.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 21 citations in OpenAlex.
- Sub-stoichiometric 5-methoxyuridine modification enables tunable immune evasion and protein expression from synthetic mRNAs.Molecular therapy. Nucleic acids · 2026Article
- A comprehensive analysis of the use of nucleoside analogues in RNA therapeutics.Journal of translational medicine · 2026Review
- Genetic Engineering of Human Keratinocytes Using CRISPR/Cas9 Ribonucleoprotein Complexes or Modified Cas9-Encoding mRNAs.Methods in molecular biology (Clifton, N.J.) · 2026Article
- On the Importance of Halogen and Chalcogen Bonds in the Solid State of Nucleic Acids: A Combined Crystallographic and Theoretical Perspective.International journal of molecular sciences · 2023Article
- Membrane linked RNA glycosylation as new trend to envision epi-transcriptome epoch.Cancer gene therapy · 2023Review
- On the mechanism of RNA phosphodiester backbone cleavage in the absence of solvent.Nucleic acids research · 2015Article
- Synthesis and optical behaviors of 6-seleno-deoxyguanosine.Science China. Chemistry · 2014Article
- Novel complex MAD phasing and RNase H structural insights using selenium oligonucleotides.Acta crystallographica. Section D, Biological crystallography · 2014Article
- Structural insights of non-canonical U*U pair and Hoogsteen interaction probed with Se atom.Nucleic acids research · 2013Article
- Synthesis of 6-Se-guanosine RNAs for structural study.Organic letters · 2013Article
- Synthesis of novel di-Se-containing thymidine and Se-DNAs for structure and function studies.Science China. Chemistry · 2013Article
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
Natural RNAs, especially tRNAs, are extensively modified to tailor structure and function diversities. Uracil is the most modified nucleobase among all natural nucleobases. Interestingly, >76% of uracil modifications are located on its 5-position. We have investigated the natural 5-methoxy (5-O-CH(3)) modification of uracil in the context of A-form oligonucleotide duplex. Our X-ray crystal structure indicates first a H-bond formation between the uracil 5-O-CH(3) and its 5'-phosphate. This novel H-bond is not observed when the oxygen of 5-O-CH(3) is replaced with a larger atom (selenium or sulfur). The 5-O-CH(3) modification does not cause significant structure and stability alterations. Moreover, our computational study is consistent with the experimental observation. The investigation on the uracil 5-position demonstrates the importance of this RNA modification at the atomic level. Our finding suggests a general interaction between the nucleobase and backbone and reveals a plausible function of the tRNA 5-O-CH(3) modification, which might potentially rigidify the local conformation and facilitates translation.
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