Evidence map›Paper›PMID 22641388›Full record

ArticleNanotechnology2012

Electromechanical and elastic probing of bacteria in a cell culture medium.

G L Thompson, V V Reukov, M P Nikiforov, S Jesse, S V Kalinin, A A Vertegel

Abstract read
In one paragraph

Article in Nanotechnology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.4field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

G L ThompsonDepartment of Bioengineering, Clemson University, Clemson, SC 29634, USA.
V V Reukov
M P Nikiforov
S Jesse
S V Kalinin
A A Vertegel
Clemson University · USOak Ridge National Laboratory · USArgonne National Laboratory · US

Funding

Electromechanical Imaging of Live Vascular Smooth Muscle CellsR21RR024449 · NCRR · CLEMSON UNIVERSITY · PI VERTEGEL, ALEXEY A · 2009 to 2011
$561k
NCRR NIH HHS R21 RR024449NCRR NIH HHS RR024449
6 · The paper itself

Abstract

Rapid phenotype characterization and identification of cultured cells, which is needed for progress in tissue engineering and drug testing, requires an experimental technique that measures physical properties of cells with sub-micron resolution. Recently, band excitation piezoresponse force microscopy (BEPFM) has been proven useful for recognition and imaging of bacteria of different types in pure water. Here, the BEPFM method is performed for the first time on physiologically relevant electrolyte media, such as Dulbecco's phosphate-buffered saline (DPBS) and Dulbecco's modified Eagle's medium (DMEM). Distinct electromechanical responses for Micrococcus lysodeikticus (Gram-positive) and Pseudomonas fluorescens (Gram-negative) bacteria in DPBS are demonstrated. The results suggest that mechanical properties of the outer surface coating each bacterium, as well as the electrical double layer around them, are responsible for the BEPFM image formation mechanism in electrolyte media.

Indexed as

BacteriaBacterial Typing TechniquesBiomechanical PhenomenaCulture MediaElasticityElectrolytesMicrococcusMicroscopyPhenotypePolylysinePseudomonas fluorescensWaterCulture MediaElectrolytesPolylysineWater

Identifiers

PMID22641388
PMCPMC3409894
OpenAlexW2017597926

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.