ArticleMicrobial cell factories2012
Production of 2,3-butanediol in Saccharomyces cerevisiae by in silico aided metabolic engineering.
Article in Microbial cell factories, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
50 citing papers in PubMed, 1 synthesis or guideline pooled it, 161 citations in OpenAlex.
- Pooled it
- Effect of Nitrosative Stress on Biofilm Formation and Upregulation of 2,3-Butanediol Dehydrogenase in Saccharomyces Cerevisiae.Current microbiology · 2025Article
- RBI: a novel algorithm for regulatory-metabolic network model in designing the optimal mutant strain.PeerJ. Computer science · 2025Article
- Chemical and Sensory Profile of Grape Distillates Aged inMolecules (Basel, Switzerland) · 2024Article
- Perturbations in mitochondrial metabolism associated with defective cardiolipin biosynthesis: AnbioRxiv : the preprint server for biology · 2024Article
- Snapshot of the Probiotic Potential ofFoods (Basel, Switzerland) · 2023Article
- Improved 2,3-Butanediol Production Rate of Metabolically EngineeredInternational journal of molecular sciences · 2023Article
- Sustainable metabolic engineering requires a perfect trifecta.Current opinion in biotechnology · 2023Review
- Identification of Bacterial Metabolites Modulating Breast Cancer Cell Proliferation and Epithelial-Mesenchymal Transition.Molecules (Basel, Switzerland) · 2023Article
- A highly efficient transcriptome-based biosynthesis of non-ethanol chemicals in Crabtree negative Saccharomyces cerevisiae.Biotechnology for biofuels and bioproducts · 2023Article
- Systematizing Microbial Bioplastic Production for Developing Sustainable Bioeconomy: Metabolic Nexus Modeling, Economic and Environmental Technologies Assessment.Journal of polymers and the environment · 2023Review
- Development of an industrial yeast strain for efficient production of 2,3-butanediol.Microbial cell factories · 2022Article
- In Silico Design Strategies for the Production of Target Chemical Compounds Using Iterative Single-Level Linear Programming Problems.Biomolecules · 2022Article
- Bioengineering for the industrial production of 2,3-butanediol by the yeast, Saccharomyces cerevisiae.World journal of microbiology & biotechnology · 2022Review
- Brewing and biochemical characterization ofFood production, processing and nutrition · 2022Article
- Diversity and sex differences in rectal gland volatiles of Queensland fruit fly, Bactrocera tryoni (Diptera: Tephritidae).PloS one · 2022Article
- Microbial production of advanced biofuels.Nature reviews. Microbiology · 2021Review
- Evaluation of 2,3-Butanediol Production from Red SeaweedJournal of microbiology and biotechnology · 2020Article
- Adaptive laboratory evolution for growth coupled microbial production.World journal of microbiology & biotechnology · 2020Review
- Reconstruction and analysis of genome-scale metabolic model of weak Crabtree positive yeast Lachancea kluyveri.Scientific reports · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
background2,3-Butanediol is a chemical compound of increasing interest due to its wide applications. It can be synthesized via mixed acid fermentation of pathogenic bacteria such as Enterobacter aerogenes and Klebsiella oxytoca. The non-pathogenic Saccharomyces cerevisiae possesses three different 2,3-butanediol biosynthetic pathways, but produces minute amount of 2,3-butanediol. Hence, we attempted to engineer S. cerevisiae strain to enhance 2,3-butanediol production.
resultsWe first identified gene deletion strategy by performing in silico genome-scale metabolic analysis. Based on the best in silico strategy, in which disruption of alcohol dehydrogenase (ADH) pathway is required, we then constructed gene deletion mutant strains and performed batch cultivation of the strains. Deletion of three ADH genes, ADH1, ADH3 and ADH5, increased 2,3-butanediol production by 55-fold under microaerobic condition. However, overproduction of glycerol was observed in this triple deletion strain. Additional rational design to reduce glycerol production by GPD2 deletion altered the carbon fluxes back to ethanol and significantly reduced 2,3-butanediol production. Deletion of ALD6 reduced acetate production in strains lacking major ADH isozymes, but it did not favor 2,3-butanediol production. Finally, we introduced 2,3-butanediol biosynthetic pathway from Bacillus subtilis and E. aerogenes to the engineered strain and successfully increased titer and yield. Highest 2,3-butanediol titer (2.29 . l-1) and yield (0.113 g . g-1) were achieved by Δadh1 Δadh3 Δadh5 strain under anaerobic condition.
conclusionsWith the aid of in silico metabolic engineering, we have successfully designed and constructed S. cerevisiae strains with improved 2,3-butanediol production.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.