ArticleScandinavian journal of rheumatology2012
MALDI MS imaging as a powerful tool for investigating synovial tissue.
Article in Scandinavian journal of rheumatology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 50 citations in OpenAlex.
- Tissue and extracellular matrix remodeling of the subchondral bone during osteoarthritis of knee joints as revealed by spatial mass spectrometry imaging.Bone research · 2026Article
- Fatty Acid Metabolic Reprogramming in Rheumatoid Arthritis: Integrating Inflammation, Resolution Failure, and Joint Destruction.Journal of inflammation research · 2026Review
- Proteomics appending a complementary dimension to precision oncotherapy.Computational and structural biotechnology journal · 2024Review
- Spatial analysis of the osteoarthritis microenvironment: techniques, insights, and applications.Bone research · 2024Review
- Biological injection therapy with leukocyte-poor platelet-rich plasma induces cellular alterations, enhancement of lubricin, and inflammatory downregulationFrontiers in surgery · 2023Article
- A Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Assay Identifies Nilotinib as an Inhibitor of Inflammation in Acute Myeloid Leukemia.Journal of medicinal chemistry · 2022Article
- Typical response of CD14Frontiers in medicine · 2022Article
- Biomarkers of Joint Damage in Osteoarthritis: Current Status and Future Directions.Mediators of inflammation · 2021Review
- Mass Spectrometry Imaging as a Potential Tool to Investigate Human Osteoarthritis at the Tissue Level.International journal of molecular sciences · 2020Review
- Neuropeptides: important regulators of joint homeostasis.Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA · 2019Review
- Imaging mass spectrometry in drug development and toxicology.Archives of toxicology · 2017Review
- Mass spectrometry imaging: a novel technology in rheumatology.Nature reviews. Rheumatology · 2017Review
- Reliable Entity Subtyping in Non-small Cell Lung Cancer by Matrix-assisted Laser Desorption/Ionization Imaging Mass Spectrometry on Formalin-fixed Paraffin-embedded Tissue Specimens.Molecular & cellular proteomics : MCP · 2016Article
- Is there a role of synovial biopsy in drug development?BMC musculoskeletal disorders · 2016Review
- [MALDI mass spectrometry of the meniscus. Objectification of morphological findings].Zeitschrift fur Rheumatologie · 2015Article
- Investigation of neutrophilic peptides in periprosthetic tissue by matrix-assisted laser desorption ionisation time-of-flight imaging mass spectrometry.International orthopaedics · 2015Article
- [Molecular pathological diagnostics of infections in orthopedic pathology].Der Pathologe · 2014Review
- Emerging mass spectrometry techniques for the direct analysis of microbial colonies.Current opinion in microbiology · 2014Article
- Matrix assisted laser desorption ionization mass spectrometry imaging identifies markers of ageing and osteoarthritic cartilage.Arthritis research & therapy · 2014Article
- Challenges and recent advances in mass spectrometric imaging of neurotransmitters.Bioanalysis · 2014Review
Corrections and comments
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Authors and funding
13 authors at 5 institutions in 2 countries.
Funding
Abstract
objectiveTo identify and image protein biomarker candidates in the synovial tissue of patients with rheumatoid arthritis (RA) and patients with osteoarthritis (OA).
methodsA novel matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) technique was applied to the analysis of synovial tissue. Patients were classified according to the American College of Rheumatology (ACR) criteria for RA. Frozen sections were stained to obtain morphological data. Serial sections were desiccated, and spotted with matrix for MALDI analysis. Ions generated by laser irradiation of the tissue were separated in time, based on their m/z ratio, and were subsequently detected. IMS was used in a 'profiling' mode to detect discrete spots for rapid evaluation of proteomic patterns in various tissue compartments. Photomicrographs of the stained tissue images were reviewed by a pathologist. Areas of interest (10 discrete areas/compartment) were marked digitally and the histology-annotated images were merged to form a photomicrograph of the section taken before the MALDI measurement. Pixel coordinates of these areas were transferred to a robotic spotter, the matrix was spotted, and the coordinates of the spots were transferred to a mass spectrometer for spectral acquisition. The data generated were then subjected to biocomputation analysis to reveal the biomarker candidates.
resultsSeveral peaks (m/z) consistent in mass with calgranulins, defensins, and thymosins were detected and their distribution in various synovial compartments (synovial lining and sublining layer) was demonstrated.
conclusionMALDI IMS is a powerful tool for the rapid detection of numerous proteins (in situ proteomics) and was applied here for the analysis of the distribution of proteins in synovial tissue sections.
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