Evidence map›Paper›PMID 22639195›Full record

ArticleJournal of biomolecular NMR2012

A large geometric distortion in the first photointermediate of rhodopsin, determined by double-quantum solid-state NMR.

Maria Concistrè, Ole G Johannessen, Neville McLean, Petra H M Bovee-Geurts, Richard C D Brown, Willem J Degrip, Malcolm H Levitt

Abstract read
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Article in Journal of biomolecular NMR, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Maria ConcistrèSchool of Chemistry, University of Southampton, SO17 1BJ, Southampton, UK. mariac@soton.ac.uk
Ole G Johannessen
Neville McLean
Petra H M Bovee-Geurts
Richard C D Brown
Willem J Degrip
Malcolm H Levitt

Funding

Biotechnology and Biological Sciences Research Council BBS/B/04382
6 · The paper itself

Abstract

Double-quantum magic-angle-spinning NMR experiments were performed on 11,12-(13)C(2)-retinylidene-rhodopsin under illumination at low temperature, in order to characterize torsional angle changes at the C11-C12 photoisomerization site. The sample was illuminated in the NMR rotor at low temperature (~120 K) in order to trap the primary photointermediate, bathorhodopsin. The NMR data are consistent with a strong torsional twist of the HCCH moiety at the isomerization site. Although the HCCH torsional twist was determined to be at least 40°, it was not possible to quantify it more closely. The presence of a strong twist is in agreement with previous Raman observations. The energetic implications of this geometric distortion are discussed.

Indexed as

Models, MolecularNuclear Magnetic Resonance, BiomolecularQuantum TheoryRhodopsinTemperaturebathorhodopsinRhodopsin

Identifiers

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.