ArticleThe Journal of membrane biology2012
Electric field exposure triggers and guides formation of pseudopod-like blebs in U937 monocytes.
Article in The Journal of membrane biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 39 citations in OpenAlex.
- Electroporation and Electrochemotherapy in Gynecological and Breast Cancer Treatment.Molecules (Basel, Switzerland) · 2022Review
- Nanosecond Pulsed Electric Field Only Transiently Affects the Cellular and Molecular Processes of Leydig Cells.International journal of molecular sciences · 2021Article
- Cytoskeletal Disruption after Electroporation and Its Significance to Pulsed Electric Field Therapies.Cancers · 2020Review
- Mechanisms and immunogenicity of nsPEF-induced cell death in B16F10 melanoma tumors.Scientific reports · 2019Article
- Characterization of Cell Membrane Permeability In Vitro Part II: Computational Model of Electroporation-Mediated Membrane Transport.Technology in cancer research & treatment · 2018Article
- Electrosensitization Increases Antitumor Effectiveness of Nanosecond Pulsed Electric FieldsTechnology in cancer research & treatment · 2017Article
- Activation of the phospholipid scramblase TMEM16F by nanosecond pulsed electric fields (nsPEF) facilitates its diverse cytophysiological effects.The Journal of biological chemistry · 2017Article
- Selective susceptibility to nanosecond pulsed electric field (nsPEF) across different human cell types.Cellular and molecular life sciences : CMLS · 2017Article
- Effect of Cooling On Cell Volume and Viability After Nanoelectroporation.The Journal of membrane biology · 2017Article
- The cytotoxic synergy of nanosecond electric pulses and low temperature leads to apoptosis.Scientific reports · 2016Article
- Involvement of Membrane Blebbing in Immunological Disorders and Cancer.Medical principles and practice : international journal of the Kuwait University, Health Science Centre · 2016Review
- Lamellipodia and Membrane Blebs Drive Efficient Electrotactic Migration of Rat Walker Carcinosarcoma Cells WC 256.PloS one · 2016Article
- Disassembly of actin structures by nanosecond pulsed electric field is a downstream effect of cell swelling.Bioelectrochemistry (Amsterdam, Netherlands) · 2014Article
- Calcium-mediated pore expansion and cell death following nanoelectroporation.Biochimica et biophysica acta · 2014Article
- Cellular regulation of extension and retraction of pseudopod-like blebs produced by nanosecond pulsed electric field (nsPEF).Cell biochemistry and biophysics · 2014Article
- Nanosecond pulsed electric field stimulation of reactive oxygen species in human pancreatic cancer cells is Ca(2+)-dependent.Biochemical and biophysical research communications · 2013Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We describe a new phenomenon of anodotropic pseudopod-like blebbing in U937 cells stimulated by nanosecond pulsed electric field (nsPEF). In contrast to "regular," round-shaped blebs, which are often seen in response to cell damage, pseudopod-like blebs (PLBs) formed as longitudinal membrane protrusions toward anode. PLB length could exceed the cell diameter in 2 min of exposure to 60-ns, 10-kV/cm pulses delivered at 10-20 Hz. Both PLBs and round-shaped nsPEF-induced blebs could be efficiently inhibited by partial isosmotic replacement of bath NaCl for a larger solute (sucrose), thereby pointing to the colloid-osmotic water uptake as the principal driving force for bleb formation. In contrast to round-shaped blebs, PLBs retracted within several minutes after exposure. Cells treated with 1 nM of the actin polymerization blocker cytochalasin D were unable to form PLBs and instead produced stationary, spherical blebs with no elongation or retraction capacity. Live cell fluorescent actin tagging showed that during elongation actin promptly entered the PLB interior, forming bleb cortex and scaffold, which was not seen in stationary blebs. Overall, PLB formation was governed by both passive (physicochemical) effects of membrane permeabilization and active cytoskeleton assembly in the living cell. To a certain extent, PLB mimics the membrane extension in the process of cell migration and can be employed as a nonchemical model for studies of cytomechanics, membrane-cytoskeleton interaction and cell motility.
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Registered trials
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