ArticleParasitology research2012
RNAi silencing of type V collagen in Schistosoma japonicum affects parasite morphology, spawning, and hatching.
Article in Parasitology research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 41 citations in OpenAlex.
- Evaluating the pathogenic and immunological effects of ds-GFP as a control in in vivo RNA interference studies of Schistosoma japonicum.Parasite (Paris, France) · 2025Article
- Molecular and functional characterization of Schistosoma japonicum annexin A13.Veterinary research · 2023Article
- Defining an optimal control for RNAi experiments with adult Schistosoma mansoni.Scientific reports · 2023Article
- Trichinella spiralis: Knockdown of gamma interferon inducible lysosomal thiol reductase (GILT) results in the reduction of worm burden.PLoS neglected tropical diseases · 2021Article
- RNAi-mediated silencing of Trichinella spiralis serpin-type serine protease inhibitors results in a reduction in larval infectivity.Veterinary research · 2020Article
- Molecular characterization of a Trichinella spiralis aspartic protease and its facilitation role in larval invasion of host intestinal epithelial cells.PLoS neglected tropical diseases · 2020Article
- Haemonchus contortus: siRNA mediated knockdown of matrix metalloproteinase 12A (MMP-12) results in reduction of infectivity.Parasites & vectors · 2020Article
- Oncomelania hupensis retains its ability to transmit Schistosoma japonicum 13 years after migration from permissive to non-permissive areas.Parasites & vectors · 2020Article
- Functional analysis of the Frzb2 gene in Schistosoma japonicum.Veterinary research · 2019Article
- Influence of Schistosoma japonicum programmed cell death protein 10 on the growth and development of schistosomula.Parasites & vectors · 2018Article
- Function of Nanos1 gene in the development of reproductive organs of Schistosoma japonicum.Parasitology research · 2017Article
- The siRNA-mediated silencing of Trichinella spiralis nudix hydrolase results in reduction of larval infectivity.Parasitology research · 2015Article
- RNAi-mediated silencing of enolase confirms its biological importance in Clonorchis sinensis.Parasitology research · 2014Article
- Distribution of lethal giant larvae (Lgl) protein in the tegument and negative impact of siRNA-based gene silencing on worm surface structure and egg hatching in Schistosoma japonicum.Parasitology research · 2014Article
- Review
- Association of spirochetal infection with Morgellons disease.F1000Research · 2013Article
- Applications of RNA Interference in Schistosomiasis: Gene Function Identification and Development of New Therapies.ISRN parasitology · 2013Review
- New frontiers in schistosoma genomics and transcriptomics.Journal of parasitology research · 2012Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Type V collagen is a component of non-cartilaginous tissues and is important in the determination of fibril structure and matrix organization, although its functions are still poorly understood. In this report, RNA interference (RNAi) approaches were used to investigate the effects of knockdown of the schistosome type V collagen (SjColV) gene. In this study, three different short interfering (si) RNAs targeting different regions of the gene were designed to suppress the expression of SjColV in Schistosoma japonicum using a soaking method. By establishing controls for measuring off-target RNAi effects, we found that different siRNA sequences had different levels of effectiveness. Although all the siRNAs tested reduced SjColV transcript levels, the S1 siRNA consistently reduced SjColV expression to >99 % of the control. In the following experiments, S1 siRNA was adapted to inhibit SjColV expression, and the silencing effects were detected by real-time PCR and Western blot. The spawning and egg hatching of parasites were calculated, while the worms' morphology was taken by scanning electron microscopy. The results show that silencing the expression of SjColV significantly affects the spawning and egg hatching of S. japonicum, and it also affects the worms' morphology.
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