ArticleJournal of industrial microbiology & biotechnology2012
Engineering of Rhodococcus cell catalysts for tolerance improvement by sigma factor mutation and active plasmid partition.
Article in Journal of industrial microbiology & biotechnology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Adaptive cargo deletion as an evolutionary dead-end during phage-based directed evolution.BMC research notes · 2026Article
- Tsrp1 Is a Novel Cyclic Diguanosine Monophosphate Effector that Plays a Role in the Organic-Solvent Tolerance ofJournal of microbiology and biotechnology · 2025Article
- Strategies to increase the robustness of microbial cell factories.Advanced biotechnology · 2024Review
- Improving stress tolerance and cell integrity of Rhodococcus ruber by overexpressing small-shock-protein Hsp16 of Rhodococcus.Journal of industrial microbiology & biotechnology · 2018Article
- Functional differentiation of 3-ketosteroid ΔMicrobial cell factories · 2017Article
- Ammonium acrylate biomanufacturing by an engineered Rhodococcus ruber with nitrilase overexpression and double-knockout of nitrile hydratase and amidase.Journal of industrial microbiology & biotechnology · 2016Article
- Bacterial sigma factors as targets for engineered or synthetic transcriptional control.Frontiers in bioengineering and biotechnology · 2014Review
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Authors and funding
2 authors.
Funding
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Abstract
Tolerance to various stresses is a key phenotype for cell catalysts, which are used widely in bioproduction of diverse valuable chemicals. Using the Rhodococcus ruber TH strain, which exhibits high nitrile hydratase activity, as the target cell catalyst for acrylamide production, we established a method to improve cell tolerance by stably introducing global transcription perturbation. The σ(70) gene (sigA) of R. ruber was cloned and randomly mutated. An R. ruber TH3/pNV-sigA(M) library containing additional sigA mutants was constructed and used for survival selection. The TH3/M4N1-59 mutant was selected by acrylonitrile/acrylamide double stress and exhibited a 160 % extension of the half-life of nitrile hydratase upon exposure to 40 % acrylamide. A redesigned parDE(M) gene was introduced to Rhodococcus to accomplish stable inheritance of plasmids. A two-batch acrylonitrile hydration reaction was performed using the engineered cells as a catalyst. Compared to TH3, the acrylamide productivity of TH3/M4N1-59DE(M) catalysis increased by 27.8 and 37.5 % in the first and second bioreaction batches, respectively. These data suggest a novel method for increasing the bioconversion productivity of target chemicals through sigA mutation of the cell catalyst.
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