ArticleThe Journal of biological chemistry2012
Subunit Interactions and cooperativity in the microtubule-severing AAA ATPase spastin.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Genotype-structure-phenotype correlations define divergent natural history in early-onset spastic paraplegia type 4.Brain : a journal of neurology · 2026Article
- Microtubule lattice defects facilitate spastin-mediated severing.Journal of cell science · 2026Article
- Article
- FBXL17/spastin axis as a novel therapeutic target of hereditary spastic paraplegia.Cell & bioscience · 2022Article
- In Vitro Reconstitution of Microtubule Dynamics and Severing Imaged by Label-Free Interference-Reflection Microscopy.Methods in molecular biology (Clifton, N.J.) · 2022Article
- The Role of Spastin in Axon Biology.Frontiers in cell and developmental biology · 2022Review
- Cutting, Amplifying, and Aligning Microtubules with Severing Enzymes.Trends in cell biology · 2021Review
- The molecular principles governing the activity and functional diversity of AAA+ proteins.Nature reviews. Molecular cell biology · 2020Review
- An allosteric network in spastin couples multiple activities required for microtubule severing.Nature structural & molecular biology · 2019Article
- Hereditary spastic paraplegia: gain-of-function mechanisms revealed by new transgenic mouse.Human molecular genetics · 2019Article
- Microtubule-severing enzymes: From cellular functions to molecular mechanism.The Journal of cell biology · 2018Review
- Functional differences of short and long isoforms of spastin harboring missense mutation.Disease models & mechanisms · 2018Article
- From structure to mechanism-understanding initiation of DNA replication.Genes & development · 2017Review
- Assessing heterogeneity in oligomeric AAA+ machines.Cellular and molecular life sciences : CMLS · 2017Review
- Article
- Katanin Severing and Binding Microtubules Are Inhibited by Tubulin Carboxy Tails.Biophysical journal · 2015Article
- Hereditary spastic paraplegia SPG4: what is known and not known about the disease.Brain : a journal of neurology · 2015Review
- N-terminal phosphorylation of p60 katanin directly regulates microtubule severing.Journal of molecular biology · 2013Article
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7 authors.
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Abstract
Spastin is a hexameric ring AAA ATPase that severs microtubules. To see whether the ring complex funnels the energy of multiple ATP hydrolysis events to the site of mechanical action, we investigate here the cooperativity of spastin. Several lines of evidence indicate that interactions among two subunits dominate the cooperative behavior: (i) the ATPase activity shows a sigmoidal dependence on the ATP concentration; (ii) ATPγS displays a mixed-inhibition behavior for normal ATP turnover; and (iii) inactive mutant subunits inhibit the activity of spastin in a hyperbolic dependence, characteristic for two interacting species. A quantitative model based on neighbor interactions fits mutant titration experiments well, suggesting that each subunit is mainly influenced by one of its neighbors. These observations are relevant for patients suffering from SPG4-type hereditary spastic paraplegia and explain why single amino acid exchanges lead to a dominant negative phenotype. In severing assays, wild type spastin is even more sensitive toward the presence of inactive mutants than in enzymatic assays, suggesting a weak coupling of ATPase and severing activity.
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