ArticleApplied and environmental microbiology2012
Real-time PCR methodology for selective detection of viable Escherichia coli O157:H7 cells by targeting Z3276 as a genetic marker.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 62 citations in OpenAlex.
- Molecular characterization of multi-drug resistance Escherichia coli O157:H7 from calf feces and cow milk in Bangladesh.Scientific reports · 2026Article
- Optimization of the viability PCR for accurate detection of Staphylococcus aureus in food samples.PloS one · 2025Article
- Digital PCR Validation for Characterization of Quantitative Reference Material ofMethods and protocols · 2024Article
- Quickly assessing disinfection effectiveness to control the spread of African swine fever virus.Applied microbiology and biotechnology · 2023Article
- Point-of-Care Lateral Flow Detection of ViableFoods (Basel, Switzerland) · 2022Article
- Chicken Skin Decontamination of ThermotolerantPathogens (Basel, Switzerland) · 2022Article
- In-House Validation of Multiplex PCR for Simultaneous Detection of Shiga Toxin-ProducingFoods (Basel, Switzerland) · 2022Article
- Prospects for the application of infectious virus detection technology based on propidium monoazide in African swine fever management.Frontiers in microbiology · 2022Review
- BCIG-SMAC medium and PMA-qPCR for differential detection of viableIranian journal of microbiology · 2021Article
- An Optimized Method to Assess ViableFrontiers in microbiology · 2020Article
- The Use of a DNA-Intercalating Dye for Quantitative Detection of ViableFrontiers in microbiology · 2019Article
- An improved assay for rapid detection of viable Staphylococcus aureus cells by incorporating surfactant and PMA treatments in qPCR.BMC microbiology · 2018Article
- The Fimbrial GeneFrontiers in microbiology · 2018Article
- Article
- Presence and Persistence ofFrontiers in public health · 2018Review
- Multiplex real-time PCR assay for detection of Escherichia coli O157:H7 and screening for non-O157 Shiga toxin-producing E. coli.BMC microbiology · 2017Article
- Recent developments in detection and enumeration of waterborne bacteria: a retrospective minireview.MicrobiologyOpen · 2016Review
- Selective detection of viable seed-borne Acidovorax citrulli by real-time PCR with propidium monoazide.Scientific reports · 2016Article
- Advances and Challenges in Viability Detection of Foodborne Pathogens.Frontiers in microbiology · 2016Review
- Development of a Sandwich ELISA for EHEC O157:H7 Intimin γ1.PloS one · 2016Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The goal of this study was to develop a sensitive, specific, and accurate method for the selective detection of viable Escherichia coli O157:H7 cells in foods. A unique open reading frame (ORF), Z3276, was identified as a specific genetic marker for the detection of E. coli O157:H7. We developed a real-time PCR assay with primers and probe targeting ORF Z3276 and confirmed that this assay was sensitive and specific for E. coli O157:H7 strains (n = 298). Using this assay, we can detect amounts of genomic DNA of E. coli O157:H7 as low as a few CFU equivalents. Moreover, we have developed a new propidium monoazide (PMA)-real-time PCR protocol that allows for the clear differentiation of viable from dead cells. In addition, the protocol was adapted to a 96-well plate format for easy and consistent handling of a large number of samples. Amplification of DNA from PMA-treated dead cells was almost completely inhibited, in contrast to the virtually unaffected amplification of DNA from PMA-treated viable cells. With beef spiked simultaneously with 8 × 10(7) dead cells/g and 80 CFU viable cells/g, we were able to selectively detect viable E. coli O157:H7 cells with an 8-h enrichment. In conclusion, this PMA-real-time PCR assay offers a sensitive and specific means to selectively detect viable E. coli O157:H7 cells in spiked beef. It also has the potential for high-throughput selective detection of viable E. coli O157:H7 cells in other food matrices and, thus, will have an impact on the accurate microbiological and epidemiological monitoring of food safety and environmental sources.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.