ArticleNature nanotechnology2012
Cell-free protein synthesis and assembly on a biochip.
Article in Nature nanotechnology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed.
- On-Chip De Novo Production of mRNA Vaccine in Lipid Nanoparticles.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Biofunctionalized patterned platform as microarray biochip to supervise delivery and expression of pDNA nanolipoplexes in stem cells via mechanotransduction.Journal of nanobiotechnology · 2025Article
- Cell-Free Gene Expression: Methods and Applications.Chemical reviews · 2025Review
- Cell-Free Gene Expression in Bioprinted Fluidic Networks.ACS synthetic biology · 2024Article
- Article
- Programming multi-protein assembly by gene-brush patterns and two-dimensional compartment geometry.Nature nanotechnology · 2020Article
- Autonomous synthesis and assembly of a ribosomal subunit on a chip.Science advances · 2020Article
- Cell-free gene expression: an expanded repertoire of applications.Nature reviews. Genetics · 2020Review
- Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography.Journal of visualized experiments : JoVE · 2018Article
- High-throughput screening of biomolecules using cell-free gene expression systems.Synthetic biology (Oxford, England) · 2018Review
- Cell-free synthetic biology forBiochemical Society transactions · 2017Review
- DNA condensation in one dimension.Nature nanotechnology · 2016Article
- Cell-Free Synthetic Biology: Engineering Beyond the Cell.Cold Spring Harbor perspectives in biology · 2016Review
- Implementation of cell-free biological networks at steady state.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Entropy-driven collective interactions in DNA brushes on a biochip.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Article
- Label-free detection with high-Q microcavities: a review of biosensing mechanisms for integrated devices.Nanophotonics (Berlin, Germany) · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biologically active complexes such as ribosomes and bacteriophages are formed through the self-assembly of proteins and nucleic acids. Recapitulating these biological self-assembly processes in a cell-free environment offers a way to develop synthetic biodevices. To visualize and understand the assembly process, a platform is required that enables simultaneous synthesis, assembly and imaging at the nanoscale. Here, we show that a silicon dioxide grid, used to support samples in transmission electron microscopy, can be modified into a biochip to combine in situ protein synthesis, assembly and imaging. Light is used to pattern the biochip surface with genes that encode specific proteins, and antibody traps that bind and assemble the nascent proteins. Using transmission electron microscopy imaging we show that protein nanotubes synthesized on the biochip surface in the presence of antibody traps efficiently assembled on these traps, but pre-assembled nanotubes were not effectively captured. Moreover, synthesis of green fluorescent protein from its immobilized gene generated a gradient of captured proteins decreasing in concentration away from the gene source. This biochip could be used to create spatial patterns of proteins assembled on surfaces.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.