ArticleThe Plant cell2012
PHO2-dependent degradation of PHO1 modulates phosphate homeostasis in Arabidopsis.
Article in The Plant cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 166 papers.
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Who cites it
166 citing papers in PubMed, 364 citations in OpenAlex.
- CsSPX-PHR/PHL-PHT1 and CsmiR399a-PHO2-PHT1/PHO1 Modules Orchestrate Pi Use Efficiency and Aluminium Tolerance in Tea Plants.Plant, cell & environment · 2026Article
- The PHO1 protein family in phosphate export and beyond.Plant physiology · 2026Review
- The transcription factor PdePHR1 increases phosphorus accumulation in poplar shoot and promotes root growth.BMC plant biology · 2026Article
- Medicago phosphate exporter PHO1.3 regulates arbuscular mycorrhizal symbiosis.BMC plant biology · 2026Article
- Transcriptome profiling of Rehmannia glutinosa uncovers ABA/GA antagonism mechanisms in tuberous root initiation.Functional & integrative genomics · 2026Article
- PHR-Mediated Pi Starvation Response Mobile Messenger RNAs Represent Noncoding Transcripts in Recipient Tissues.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Adaptation mechanisms of low-phosphorus stress in plants: physiological responses, molecular regulation, and future perspectives.Frontiers in plant science · 2026Review
- Role of Apyrase in Mobilization of Phosphate from Extracellular Nucleotides and in Regulating Phosphate Uptake in Arabidopsis.International journal of molecular sciences · 2025Article
- Regulation of maize root growth by local phosphorus availability, sucrose metabolism, and partitioning.Annals of botany · 2025Article
- Concentration-Dependent N-P Interactions Cause Organ-Specific Responses and Nutrient Allocation in Poplar Seedlings.Plants (Basel, Switzerland) · 2025Article
- Overexpression of miR399d impairs arbuscular mycorrhizal symbiosis in tomato.Plant biotechnology journal · 2025Article
- The Role of MicroRNA-Based Strategies in Optimizing Plant Biomass Composition for Bio-Based Packaging Materials.Plants (Basel, Switzerland) · 2025Review
- Organ-specific transcriptional and metabolic adaptations of potato plants to limited phosphate availability prior and after tuberization.The Plant journal : for cell and molecular biology · 2025Article
- C4 plants respond to phosphate starvation differently than C3 plants.Plant physiology · 2025Article
- Enhancing inositol pyrophosphate accumulation in plants alters growth, phosphate homeostasis, and insect herbivory.The Plant journal : for cell and molecular biology · 2025Article
- Genome-wide identification, expression analysis, and response to abiotic stress of the phosphate transporter gene family in cucumber (Cucumis sativus L.).BMC plant biology · 2025Article
- Genome-wide identification and analysis of phosphate utilization related genes (PURs) reveal their roles involved in low phosphate responses in Brassica napus L.BMC plant biology · 2025Article
- MicroRNA gatekeepers: Orchestrating rhizospheric dynamics.Journal of integrative plant biology · 2025Review
- Genome-Wide Identification and Expression Analysis of the Phosphate Transporter Gene Family inInternational journal of molecular sciences · 2025Article
- Transcriptomic Response of White Lupin Roots to Short-Term Sucrose Treatment.Plants (Basel, Switzerland) · 2025Article
106 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Arabidopsis thaliana pho2 mutant, which is defective in a ubiquitin-conjugating E2 enzyme, displays inorganic phosphate (Pi) toxicity as a result of enhanced uptake and root-to-shoot translocation of Pi. To elucidate downstream components of the PHO2-dependent regulatory pathway, we identified two pho2 suppressors as carrying missense mutations in PHO1, which has been implicated in Pi loading to the xylem. In support of the genetic interaction between PHO1 and PHO2, we found that the protein level of PHO1 is increased in pho2, whereas such accumulation is ameliorated in both pho2 suppressors. Results from cycloheximide and endosomal Cys protease inhibitor E-64d treatments further suggest that PHO1 degradation is PHO2 dependent and involves multivesicular body-mediated vacuolar proteolysis. Using the transient expression system of tobacco (Nicotiana tabacum) leaves, we demonstrated that PHO1 and PHO2 are partially colocalized and physically interact in the endomembranes, where the ubiquitin conjugase activity of PHO2 is required for PHO1 degradation. In addition, reduced PHO1 expression caused by PHO1 mutations impede Pi uptake, indicating a functional association between xylem loading and acquisition of Pi. Together, our findings uncover a pivotal molecular mechanism by which PHO2 modulates the degradation of PHO1 in the endomembranes to maintain Pi homeostasis in plants.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.