ArticleJournal of neuroscience methods2012
Genetically encoded fluorescent voltage sensors using the voltage-sensing domain of Nematostella and Danio phosphatases exhibit fast kinetics.
Article in Journal of neuroscience methods, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 39 citations in OpenAlex.
- Imaging different cell populations in the mouse olfactory bulb using the genetically encoded voltage indicator ArcLight.Neurophotonics · 2024Review
- Voltage Imaging with Engineered Proton-Pumping Rhodopsins: Insights from the Proton Transfer Pathway.ACS physical chemistry Au · 2023Review
- Optical consequences of a genetically-encoded voltage indicator with a pH sensitive fluorescent protein.Neuroscience research · 2019Review
- Optimizing Strategies for Developing Genetically Encoded Voltage Indicators.Frontiers in cellular neuroscience · 2019Review
- Genetically Encoded Fluorescent Biosensors Illuminate the Spatiotemporal Regulation of Signaling Networks.Chemical reviews · 2018Review
- Voltage and Calcium Imaging of Brain Activity.Biophysical journal · 2017Review
- Improving a genetically encoded voltage indicator by modifying the cytoplasmic charge composition.Scientific reports · 2017Article
- Article
- Directed Evolution of Key Residues in Fluorescent Protein Inverses the Polarity of Voltage Sensitivity in the Genetically Encoded Indicator ArcLight.ACS chemical neuroscience · 2017Article
- Genetically Encoded Voltage Indicators: Opportunities and Challenges.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Review
- Design and development of genetically encoded fluorescent sensors to monitor intracellular chemical and physical parameters.Biophysical reviews · 2016Review
- Pado, a fluorescent protein with proton channel activity can optically monitor membrane potential, intracellular pH, and map gap junctions.Scientific reports · 2016Article
- Currents through Hv1 channels deplete protons in their vicinity.The Journal of general physiology · 2016Article
- Genetically Encoded Voltage Indicators in Circulation Research.International journal of molecular sciences · 2015Review
- Designs and sensing mechanisms of genetically encoded fluorescent voltage indicators.Current opinion in chemical biology · 2015Review
- Monitoring brain activity with protein voltage and calcium sensors.Scientific reports · 2015Article
- Computer-generated holography enhances voltage dye fluorescence discrimination in adjacent neuronal structures.Neurophotonics · 2015Article
- Linker length and fusion site composition improve the optical signal of genetically encoded fluorescent voltage sensors.Neurophotonics · 2015Article
- Combinatorial mutagenesis of the voltage-sensing domain enables the optical resolution of action potentials firing at 60 Hz by a genetically encoded fluorescent sensor of membrane potential.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Bright and fast multicoloured voltage reporters via electrochromic FRET.Nature communications · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
Abstract
A substantial increase in the speed of the optical response of genetically encoded fluorescent protein voltage sensors (FP voltage sensors) was achieved by using the voltage-sensing phosphatase genes of Nematostella vectensis and Danio rerio. A potential N. vectensis voltage-sensing phosphatase was identified in silico. The voltage-sensing domain (S1-S4) of the N. vectensis homolog was used to create an FP voltage sensor called Nema. By replacing the phosphatase with a cerulean/citrine FRET pair, a new FP voltage sensor was synthesized with fast off kinetics (Tau(off)<5ms). However, the signal was small (ΔF/F=0.4%/200mV). FP voltage sensors using the D. rerio voltage-sensing phosphatase homolog, designated Zahra and Zahra 2, exhibited fast on and off kinetics within 2ms of the time constants observed with the organic voltage-sensitive dye, di4-ANEPPS. Mutagenesis of the S4 region of the Danio FP voltage sensor shifted the voltage dependence to more negative potentials but did not noticeably affect the kinetics of the optical signal.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.