Evidence map›Paper›PMID 22633243›Full record

ArticleVirology2012

Moloney murine leukemia virus genomic RNA packaged in the absence of a full complement of wild type nucleocapsid protein.

Silas F Johnson, Eric L Garcia, Michael F Summers, Alice Telesnitsky

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Silas F JohnsonDepartment of Microbiology and Immunology, University of Michigan, Ann Arbor, MI 48109, USA.
Eric L Garcia
Michael F Summers
Alice Telesnitsky
University of Michigan · USHoward Hughes Medical Institute · US

Funding

CELLULAR AND MOLECULAR BIOLOGY AT MICHIGANT32GM007315 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI PUTHENVEEDU, MANOJKUMAR A · 1985 to 2021
$12.8M
NMR STUDIES OF RETROVIRAL NUCLEOCAPSID PROTEINSR01GM042561 · NIGMS · UNIVERSITY OF MARYLAND BALTIMORE COUNTY · PI SUMMERS, MICHAEL FINLEY · 1994 to 2019
$9.4M
Genomic Determinants of Murine Leukemia VirusR01CA069300 · NCI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI TELESNITSKY, ALICE · 2002 to 2006
$1.3M
NCI NIH HHS R01 CA069300NCI NIH HHS R01CA069300NIGMS NIH HHS R01 GM042561NIGMS NIH HHS R01GM042561NIGMS NIH HHS T32 GM007315
6 · The paper itself

Abstract

The current model for MLV genomic RNA (gRNA) packaging predicts that of the thousands of Gag proteins in a budding virion, only a small number (≤1%) may be necessary to recruit gRNA. Here, we examined the threshold limits of functional Gag required to package gRNA using wild-type (WT) and packaging deficient mutant nucleocapsid (NC) phenotypically mixed virions. Although gRNA packaging was severely diminished for the NC mutant, the residual encapsidated RNA dimer displayed motility on gels, thermostability, and integrity that was indistinguishable from that of WT. In phenotypically mixed virions, gRNA encapsidation recovered to within approximately two-fold of WT levels when the amount of WT NC was 5-10% of the total. Our results demonstrate that NC's roles in gRNA dimerization and packaging are genetically separable. Additionally, MLV gRNA packaging does not require 100% WT NC, but the amount of functional NC required is greater than the predicted minimum.

Indexed as

Genome, Viral3T3 CellsAnimalsCell LineGene Products, gagHEK293 CellsHumansMiceMoloney murine leukemia virusNucleocapsidNucleocapsid ProteinsRNA, ViralVirus AssemblyGag protein p12, moloney murine leukemia virusGene Products, gagNucleocapsid ProteinsRNA, Viral

Identifiers

PMID22633243
PMCPMC3379553
OpenAlexW1999407616

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.