ArticleJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology2012
Enhanced detection of Rift Valley fever virus using molecular assays on whole blood samples.
Article in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 2 of them syntheses that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Epidemiology of human and animal leptospirosis in Kenya: A systematic review and meta-analysis of disease occurrence, serogroup diversity and risk factors.PLoS neglected tropical diseases · 2024Pooled it
- Contemporary epidemiological data of Rift Valley fever virus in humans, mosquitoes and other animal species in Africa: A systematic review and meta-analysis.Veterinary medicine and science · 2023Pooled it
- Development of reference material from Rift Valley fever virus RNA.Virology journal · 2026Article
- RNA Editing as a General Trait of Ebolaviruses.The Journal of infectious diseases · 2023Article
- Tracking Rift Valley fever: From Mali to Europe and other countries, 2016.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2019Article
- Ebola viral load at diagnosis associates with patient outcome and outbreak evolution.The Journal of clinical investigation · 2015Article
- The Contribution of Ebola Viral Load at Admission and Other Patient Characteristics to Mortality in a Médecins Sans Frontières Ebola Case Management Centre, Kailahun, Sierra Leone, June-October 2014.The Journal of infectious diseases · 2015Article
- Countermeasure development for Rift Valley fever: deletion, modification or targeting of major virulence factorFuture virology · 2014Article
- Rift Valley fever virus structural and nonstructural proteins: recombinant protein expression and immunoreactivity against antisera from sheep.Vector borne and zoonotic diseases (Larchmont, N.Y.) · 2013Article
- A highly pathogenic new bunyavirus emerged in China.Emerging microbes & infections · 2013Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
backgroundRift Valley fever (RVF) is an emerging arthropod-borne zoonoses of global agricultural and public health importance. In December 2006, an RVF outbreak was recognized in Kenya which led to the deployment of international response laboratory teams to the area.
objectivesA field laboratory was operated in Malindi, Kenya to provide safe sample handling and molecular testing for RVF virus (RVFV) as well as selected other pathogens for differential diagnosis. STUDY
designSafe sample handling was carried out using a negative pressure flexible film isolator (glovebox) and commercial reagents to inactivate clinical specimens and purify nucleic acid. Whole blood was routinely used for diagnostic testing although paired plasma samples were also tested in select cases. Subsequently, human macrophages were tested in vitro for their susceptibility to RVFV.
resultsThe field laboratory received samples from 33 individuals and a definite laboratory diagnosis was provided in 16 of these cases. Using molecular diagnostic techniques, RVFV was more consistently detected in whole blood than in plasma samples most likely due to association of RVFV with blood cells. Subsequent in vitro studies identified macrophages as a target cell for RVFV replication.
conclusionsRVFV appears to replicate in blood cells such as macrophages. Thus, the sensitivity of molecular diagnostic testing is improved if whole blood is used as the clinical specimen rather than plasma or serum.
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