ArticleMalaria journal2012
Quantitative detection of PfHRP2 in saliva of malaria patients in the Philippines.
Article in Malaria journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it.
- The Assessment of the Readiness of Molecular Biomarker-Based Mobile Health Technologies for Healthcare Applications.Scientific reports · 2015Pooled it
- Noninvasive malaria detection beyond blood sampling.Sensors & diagnostics · 2026Review
- Noninvasive Approach ofBioMed research international · 2022Article
- Acceptance and perceived value of non-invasive malaria diagnostic tests in malaria-endemic countries.Malaria journal · 2021Article
- A Look Inside: Oral Sampling for Detection of Non-oral Infectious Diseases.Journal of clinical microbiology · 2021Article
- PfHRP2 detection using plasmonic optrodes: performance analysis.Malaria journal · 2021Article
- Evaluating the potential of using urine and saliva specimens for malaria diagnosis in suspected patients in Ghana.Malaria journal · 2020Article
- Microneedle-based skin patch for blood-free rapid diagnostic testing.Microsystems & nanoengineering · 2020Article
- Ultrasensitive and label-free biosensor for the detection of Plasmodium falciparum histidine-rich protein II in saliva.Scientific reports · 2019Article
- A saliva-based rapid test to quantify the infectious subclinical malaria parasite reservoir.Science translational medicine · 2019Article
- Modified Protein Misfolding Cyclic Amplification Overcomes Real-Time Quaking-Induced Conversion Assay Inhibitors in Deer Saliva To Detect Chronic Wasting Disease Prions.Journal of clinical microbiology · 2018Article
- Asymptomatic Malaria Infection and Associated Factors among Blood Donors Attending Arba Minch Blood Bank, Southwest Ethiopia.Ethiopian journal of health sciences · 2018Article
- Diagnostic tools in childhood malaria.Parasites & vectors · 2018Review
- Unclear association between levels of Plasmodium falciparum lactate dehydrogenase (PfLDH) in saliva of malaria patients and blood parasitaemia: diagnostic implications?Malaria journal · 2018Article
- Recent Progress in the Development of Diagnostic Tests for Malaria.Diagnostics (Basel, Switzerland) · 2017Review
- Enzyme-free electrochemical immunosensor based on methylene blue and the electro-oxidation of hydrazine on Pt nanoparticles.Biosensors & bioelectronics · 2017Article
- Non-invasive surveillance for Plasmodium in reservoir macaque species.Malaria journal · 2015Article
- Use of IgG in oral fluid to monitor infants with suspected congenital toxoplasmosis.Clinical and vaccine immunology : CVI · 2015Article
- Malaria diagnostics in clinical trials.The American journal of tropical medicine and hygiene · 2013Review
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMalaria is a global health priority with a heavy burden of fatality and morbidity. Improvements in field diagnostics are needed to support the agenda for malaria elimination. Saliva has shown significant potential for use in non-invasive diagnostics, but the development of off-the-shelf saliva diagnostic kits requires best practices for sample preparation and quantitative insight on the availability of biomarkers and the dynamics of immunoassay in saliva. This pilot study measured the levels of the PfHRP2 in patient saliva to inform the development of salivary diagnostic tests for malaria.
methodsMatched samples of blood and saliva were collected between January and May, 2011 from eight patients at Palawan Baptist Hospital in Roxas, Palawan, Philippines. Parasite density was determined from thick-film blood smears. Concentrations of PfHRP2 in saliva of malaria-positive patients were measured using a custom chemiluminescent ELISA in microtitre plates. Sixteen negative-control patients were enrolled at UCLA. A substantive difference between this protocol and previous related studies was that saliva samples were stabilized with protease inhibitors.
resultsOf the eight patients with microscopically confirmed P. falciparum malaria, seven tested positive for PfHRP2 in the blood using rapid diagnostic test kits, and all tested positive for PfHRP2 in saliva. All negative-control samples tested negative for salivary PfHRP2. On a binary-decision basis, the ELISA agreed with microscopy with 100 % sensitivity and 100 % specificity. Salivary levels of PfHRP2 ranged from 17 to 1,167 pg/mL in the malaria-positive group.
conclusionSaliva is a promising diagnostic fluid for malaria when protein degradation and matrix effects are mitigated. Systematic quantitation of other malaria biomarkers in saliva would identify those with the best clinical relevance and suitability for off-the-shelf diagnostic kits.
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