ArticleJournal of the American Chemical Society2012
Monitoring lipid anchor organization in cell membranes by PIE-FCCS.
Article in Journal of the American Chemical Society, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 47 citations in OpenAlex.
- Polymeric biomaterial-inspired cell surface modulation for the development of novel anticancer therapeutics.Biomaterials research · 2023Review
- Imaging Membrane Order and Dynamic Interactions in Living Cells with a DNA Zipper Probe.Angewandte Chemie (International ed. in English) · 2022Article
- Regulation of local GTP availability controls RAC1 activity and cell invasion.Nature communications · 2021Article
- Membrane anchoring facilitates colocalization of enzymes in plant cytochrome P450 redox systems.Communications biology · 2021Article
- Integrin intra-heterodimer affinity inversely correlates with integrin activatability.Cell reports · 2021Article
- Clustering of the ζ-Chain Can Initiate T Cell Receptor Signaling.International journal of molecular sciences · 2020Article
- Current Methods for Detecting Cell Membrane Transient Interactions.Frontiers in chemistry · 2020Review
- Quantifying membrane protein oligomerization with fluorescence cross-correlation spectroscopy.Methods (San Diego, Calif.) · 2018Article
- Dual-Functional Lipid Coating for the Nanopillar-Based Capture of Circulating Tumor Cells with High Purity and Efficiency.Langmuir : the ACS journal of surfaces and colloids · 2017Article
- With or without rafts? Alternative views on cell membranes.BioEssays : news and reviews in molecular, cellular and developmental biology · 2016Review
- Article
- Diffusion of GPI-anchored proteins is influenced by the activity of dynamic cortical actin.Molecular biology of the cell · 2015Article
- Class A Plexins Are Organized as Preformed Inactive Dimers on the Cell Surface.Biophysical journal · 2015Article
- E-cadherin junction formation involves an active kinetic nucleation process.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Cross-Scale Integrin Regulation Organizes ECM and Tissue Topology.Developmental cell · 2015Article
- Current approaches to studying membrane organization.F1000Research · 2015Review
- Efficient elusion of viable adhesive cells from a microfluidic system by air foam.Biomicrofluidics · 2014Article
- Time-resolved fluorescence spectroscopy measures clustering and mobility of a G protein-coupled receptor opsin in live cell membranes.Journal of the American Chemical Society · 2014Article
- Spatial organization of EphA2 at the cell-cell interface modulates trans-endocytosis of ephrinA1.Biophysical journal · 2014Article
- H-Ras forms dimers on membrane surfaces via a protein-protein interface.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
This study examines the dynamic co-localization of lipid-anchored fluorescent proteins in living cells using pulsed-interleaved excitation fluorescence cross-correlation spectroscopy (PIE-FCCS) and fluorescence lifetime analysis. Specifically, we look at the pairwise co-localization of anchors from lymphocyte cell kinase (LCK: myristoyl, palmitoyl, palmitoyl), RhoA (geranylgeranyl), and K-Ras (farnesyl) proteins in different cell types. In Jurkat cells, a density-dependent increase in cross-correlation among RhoA anchors is observed, while LCK anchors exhibit a more moderate increase and broader distribution. No correlation was detected among K-Ras anchors or between any of the different anchor types studied. Fluorescence lifetime data reveal no significant Förster resonance energy transfer in any of the data. In COS 7 cells, minimal correlation was detected among LCK or RhoA anchors. Taken together, these observations suggest that some lipid anchors take part in anchor-specific co-clustering with other existing clusters of native proteins and lipids in the membrane. Importantly, these observations do not support a simple interpretation of lipid anchor-mediated organization driven by partitioning based on binary lipid phase separation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.