ArticlePloS one2012
Actin- and dynamin-dependent maturation of bulk endocytosis restores neurotransmission following synaptic depletion.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- 25-Hydroxycholesterol modulates synaptic vesicle endocytosis at the mouse neuromuscular junction.Pflugers Archiv : European journal of physiology · 2025Article
- Nanotargeted Delivery of Immune Therapeutics in Type 1 Diabetes.Advanced materials (Deerfield Beach, Fla.) · 2023Article
- Review
- Article
- Frequency-dependent lipid raft uptake at rat diaphragm muscle axon terminals.Muscle & nerve · 2019Article
- Inhibition of endocytosis suppresses the nitric oxide-dependent release of Cl- in retinal amacrine cells.PloS one · 2018Article
- Synaptic Vesicle Endocytosis in Different Model Systems.Frontiers in cellular neuroscience · 2018Review
- Promotion of endocytosis efficiency through an ATP-independent mechanism at rat calyx of Held terminals.The Journal of physiology · 2017Article
- Myosin II activity is required for structural plasticity at the axon initial segment.The European journal of neuroscience · 2017Article
- Dynamin-1 deletion enhances post-tetanic potentiation and quantal size after tetanic stimulation at the calyx of Held.The Journal of physiology · 2017Article
- Piccolo Promotes Vesicle Replenishment at a Fast Central Auditory Synapse.Frontiers in synaptic neuroscience · 2017Article
- Tissue-specific dynamin-1 deletion at the calyx of Held decreases short-term depression through a mechanism distinct from vesicle resupply.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Presynaptic active zones in invertebrates and vertebrates.EMBO reports · 2015Review
- Small molecules demonstrate the role of dynamin as a bi-directional regulator of the exocytosis fusion pore and vesicle release.Molecular psychiatry · 2015Article
- Cholesterol regulates multiple forms of vesicle endocytosis at a mammalian central synapse.Journal of neurochemistry · 2015Article
- An acto-myosin II constricting ring initiates the fission of activity-dependent bulk endosomes in neurosecretory cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Role of Bassoon and Piccolo in Assembly and Molecular Organization of the Active Zone.Frontiers in synaptic neuroscience · 2015Review
- Article
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8 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Bulk endocytosis contributes to the maintenance of neurotransmission at the amphibian neuromuscular junction by regenerating synaptic vesicles. How nerve terminals internalize adequate portions of the presynaptic membrane when bulk endocytosis is initiated before the end of a sustained stimulation is unknown. A maturation process, occurring at the end of the stimulation, is hypothesised to precisely restore the pools of synaptic vesicles. Using confocal time-lapse microscopy of FM1-43-labeled nerve terminals at the amphibian neuromuscular junction, we confirm that bulk endocytosis is initiated during a sustained tetanic stimulation and reveal that shortly after the end of the stimulation, nerve terminals undergo a maturation process. This includes a transient bulging of the plasma membrane, followed by the development of large intraterminal FM1-43-positive donut-like structures comprising large bulk membrane cisternae surrounded by recycling vesicles. The degree of bulging increased with stimulation frequency and the plasmalemma surface retrieved following the transient bulging correlated with the surface membrane internalized in bulk cisternae and recycling vesicles. Dyngo-4a, a potent dynamin inhibitor, did not block the initiation, but prevented the maturation of bulk endocytosis. In contrast, cytochalasin D, an inhibitor of actin polymerization, hindered both the initiation and maturation processes. Both inhibitors hampered the functional recovery of neurotransmission after synaptic depletion. Our data confirm that initiation of bulk endocytosis occurs during stimulation and demonstrates that a delayed maturation process controlled by actin and dynamin underpins the coupling between exocytosis and bulk endocytosis.
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