ArticleScience (New York, N.Y.)2012
Structures from anomalous diffraction of native biological macromolecules.
Article in Science (New York, N.Y.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 101 papers.
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Who cites it
101 citing papers in PubMed.
- LEGO®-inspired electrically-actuated microfluidics for on-chip protein crystallization andLab on a chip · 2026Article
- Evolution of macromolecular crystallography beamlines at the Swiss Light Source and SwissFEL.Journal of synchrotron radiation · 2025Article
- Photoactivatable Electrophilic Glycosylselenosulfonates for Ultrafast Modification of Alkynes and Thiols.ACS central science · 2025Article
- Scalable fabrication of an array-type fixed-target device for automated room temperature X-ray protein crystallography.Scientific reports · 2025Article
- Utilizing anomalous signals for element identification in macromolecular crystallography.Acta crystallographica. Section D, Structural biology · 2024Article
- X-Ray Crystallography for Macromolecular Complexes.Advances in experimental medicine and biology · 2024Article
- Experimental phasing opportunities for macromolecular crystallography at very long wavelengths.Communications chemistry · 2023Article
- Facing the phase problem.IUCrJ · 2023Article
- Polymer-based microfluidic device for on-chip counter-diffusive crystallization andLab on a chip · 2023Article
- Structural insights into plasmalemma vesicle-associated protein (PLVAP): Implications for vascular endothelial diaphragms and fenestrae.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Article
- Native SAD phasing at room temperature.Acta crystallographica. Section D, Structural biology · 2022Article
- A crystal-processing machine using a deep-ultraviolet laser: application to long-wavelength native SAD experiments.Acta crystallographica. Section F, Structural biology communications · 2022Article
- Phosphorus and sulfur SAD phasing of the nucleic acid-bound DNA-binding domain of interferon regulatory factor 4.Acta crystallographica. Section F, Structural biology communications · 2021Article
- Structural insights into a C2 domain protein specifically found in tardigrades.Protein science : a publication of the Protein Society · 2021Article
- Synthesis and study of macrocyclic β-hairpin peptides for investigating amyloid oligomers.Methods in enzymology · 2021Article
- Article
- High-throughput in situ experimental phasing.Acta crystallographica. Section D, Structural biology · 2020Article
- Making a difference in multi-data-set crystallography: simple and deterministic data-scaling/selection methods.Acta crystallographica. Section D, Structural biology · 2020Article
- Structural basis for CaNature communications · 2020Article
41 more citing papers are in PubMed but not listed here.
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Funding
Abstract
Crystal structure analyses for biological macromolecules without known structural relatives entail solving the crystallographic phase problem. Typical de novo phase evaluations depend on incorporating heavier atoms than those found natively; most commonly, multi- or single-wavelength anomalous diffraction (MAD or SAD) experiments exploit selenomethionyl proteins. Here, we realize routine structure determination using intrinsic anomalous scattering from native macromolecules. We devised robust procedures for enhancing the signal-to-noise ratio in the slight anomalous scattering from generic native structures by combining data measured from multiple crystals at lower-than-usual x-ray energy. Using this multicrystal SAD method (5 to 13 equivalent crystals), we determined structures at modest resolution (2.8 to 2.3 angstroms) for native proteins varying in size (127 to 1148 unique residues) and number of sulfur sites (3 to 28). With no requirement for heavy-atom incorporation, such experiments provide an attractive alternative to selenomethionyl SAD experiments.
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