ArticleProceedings of the National Academy of Sciences of the United States of America2012
In vitro loading of human cohesin on DNA by the human Scc2-Scc4 loader complex.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 48 citations in OpenAlex.
- Co-essentiality analysis identifies PRR12 as a cohesin interacting protein and contributor to genomic integrity.Developmental cell · 2025Article
- PRMT5 Maintains Homeostasis of the Intestinal Epithelium by Modulating Cell Proliferation and Survival.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Regulating epithelial-mesenchymal plasticity from 3D genome organization.Communications biology · 2024Review
- Coming full circle: On the origin and evolution of the looping model for enhancer-promoter communication.The Journal of biological chemistry · 2022Review
- DDK: The Outsourced Kinase of Chromosome Maintenance.Biology · 2022Review
- Multi-phenotype analyses of hemostatic traits with cardiovascular events reveal novel genetic associations.Journal of thrombosis and haemostasis : JTH · 2022Article
- Sensitivity of cohesin-chromatin association to high-salt treatment corroborates non-topological mode of loop extrusion.Epigenetics & chromatin · 2021Article
- Identification of pathways modulating vemurafenib resistance in melanoma cells via a genome-wide CRISPR/Cas9 screen.G3 (Bethesda, Md.) · 2021Article
- Shaping of the 3D genome by the ATPase machine cohesin.Experimental & molecular medicine · 2020Review
- Analysis of clinical and genetic characteristics in 10 Chinese individuals with Cornelia de Lange syndrome and literature review.Molecular genetics & genomic medicine · 2020Review
- The cohesin loader SCC2 contains a PHD finger that is required for meiosis in land plants.PLoS genetics · 2020Article
- A streamlined cohesin apparatus is sufficient for mitosis and meiosis in the protist Tetrahymena.Chromosoma · 2018Article
- Multiple determinants and consequences of cohesion fatigue in mammalian cells.Molecular biology of the cell · 2018Article
- Brca2, Pds5 and Wapl differentially control cohesin chromosome association and function.PLoS genetics · 2018Article
- Nonreplicative functions of the origin recognition complex.Nucleus (Austin, Tex.) · 2018Review
- The torments of the cohesin ring.Nucleus (Austin, Tex.) · 2017Review
- Chl1 DNA helicase and Scc2 function in chromosome condensation through cohesin deposition.PloS one · 2017Article
- Identification of Functional Domains in the Cohesin Loader Subunit Scc4 by a Random Insertion/Dominant Negative Screen.G3 (Bethesda, Md.) · 2016Article
- Mutant cohesin affects RNA polymerase II regulation in Cornelia de Lange syndrome.Scientific reports · 2015Article
- Phosphorylation of the Scc2 cohesin deposition complex subunit regulates chromosome condensation through cohesin integrity.Molecular biology of the cell · 2015Article
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
Abstract
The loading of cohesin onto chromatin requires the heterodimeric complex sister chromatid cohesion (Scc)2 and Scc4 (Scc2/4), which is highly conserved in all species. Here, we describe the purification of the human (h)-Scc2/4 and show that it interacts with h-cohesin and the heterodimeric Smc1-Smc3 complex but not with the Smc1 or Smc3 subunit alone. We demonstrate that both h-Scc2/4 and h-cohesin are loaded onto dsDNA containing the prereplication complex (pre-RC) generated in vitro by Xenopus high-speed soluble extracts. The addition of geminin, which blocks pre-RC formation, prevents the loading of Scc2/4 and cohesin. Xenopus extracts depleted of endogenous Scc2/4 with specific antibodies, although able to form pre-RCs, did not support cohesin loading unless supplemented with purified h-Scc2/4. The results presented here indicate that the Xenopus or h-Scc2/4 complex supports the loading of Xenopus and/or h-cohesin onto pre-RCs formed by Xenopus high-speed extracts. We show that cohesin loaded onto pre-RCs either by h-Scc2/4 and/or the Xenopus complex was dissociated from chromatin by low salt extraction, similar to cohesin loaded onto chromatin in G(1) by HeLa cells in vivo. Replication of cohesin-loaded DNA, both in vitro and in vivo, markedly increased the stability of cohesin associated with DNA. Collectively, these in vitro findings partly recapitulate the in vivo pathway by which sister chromatids are linked together, leading to cohesion.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.