ArticleProceedings of the National Academy of Sciences of the United States of America2012
Engineering rotor ring stoichiometries in the ATP synthase.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 90 citations in OpenAlex.
- ATP synthesis driven by atmospheric hydrogen concentrations.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Chloroplast ATP synthase: From structure to engineering.The Plant cell · 2024Review
- Review
- Modeling control and transduction of electrochemical gradients in acid-stressed bacteria.iScience · 2023Article
- Impact of engineering the ATP synthase rotor ring on photosynthesis in tobacco chloroplasts.Plant physiology · 2023Article
- ATP synthase FCellular and molecular life sciences : CMLS · 2022Review
- Here comes the sun: How optimization of photosynthetic light reactions can boost crop yields.Journal of integrative plant biology · 2022Review
- A new class of biological ion-driven rotary molecular motors with 5:2 symmetry.Frontiers in microbiology · 2022Review
- Rotor subunits adaptations in ATP synthases from photosynthetic organisms.Biochemical Society transactions · 2021Review
- Molecular and Supramolecular Structure of the Mitochondrial Oxidative Phosphorylation System: Implications for Pathology.Life (Basel, Switzerland) · 2021Review
- Comprehensive Bioenergetic Evaluation of Microbial Pathway Variants in Syntrophic Propionate Oxidation.mSystems · 2020Article
- ATP synthase: Evolution, energetics, and membrane interactions.The Journal of general physiology · 2020Review
- Amino Acid and Sugar Catabolism in the Marine Bacterium Phaeobacter inhibens DSM 17395 from an Energetic Viewpoint.Applied and environmental microbiology · 2019Article
- Unusual features of the c-ring of FScientific reports · 2019Article
- Opposing Pressures of Speed and Efficiency Guide the Evolution of Molecular Machines.Molecular biology and evolution · 2019Article
- Mechanisms for achieving high speed and efficiency in biomolecular machines.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Heterocyst Thylakoid Bioenergetics.Life (Basel, Switzerland) · 2019Review
- Optimization of ATP Synthase c-Rings for Oxygenic Photosynthesis.Frontiers in plant science · 2019Article
- Membrane plasticity facilitates recognition of the inhibitor oligomycin by the mitochondrial ATP synthase rotor.Biochimica et biophysica acta. Bioenergetics · 2018Article
- The regulatory subunit ε in Escherichia coli FBiochimica et biophysica acta. Bioenergetics · 2018Review
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Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
ATP synthase membrane rotors consist of a ring of c-subunits whose stoichiometry is constant for a given species but variable across different ones. We investigated the importance of c/c-subunit contacts by site-directed mutagenesis of a conserved stretch of glycines (GxGxGxGxG) in a bacterial c(11) ring. Structural and biochemical studies show a direct, specific influence on the c-subunit stoichiometry, revealing c(<11), c(12), c(13), c(14), and c(>14) rings. Molecular dynamics simulations rationalize this effect in terms of the energetics and geometry of the c-subunit interfaces. Quantitative data from a spectroscopic interaction study demonstrate that the complex assembly is independent of the c-ring size. Real-time ATP synthesis experiments in proteoliposomes show the mutant enzyme, harboring the larger c(12) instead of c(11), is functional at lower ion motive force. The high degree of compliance in the architecture of the ATP synthase rotor offers a rationale for the natural diversity of c-ring stoichiometries, which likely reflect adaptations to specific bioenergetic demands. These results provide the basis for bioengineering ATP synthases with customized ion-to-ATP ratios, by sequence modifications.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.