ArticleAmerican journal of physiology. Gastrointestinal and liver physiology2012
A high-affinity and specific carrier-mediated mechanism for uptake of thiamine pyrophosphate by human colonic epithelial cells.
Article in American journal of physiology. Gastrointestinal and liver physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 42 citations in OpenAlex.
- From predisposition to recovery: field evidence of interactions between the gut microbiota and Brachyspira hyodysenteriae infection.Veterinary research · 2026Article
- Vitamin biosynthesis in the gut: interplay between mammalian host and its resident microbiota.Microbiology and molecular biology reviews : MMBR · 2025Review
- Article
- IQGAP-2: a novel interacting partner with the human colonic thiamin pyrophosphate transporter.American journal of physiology. Cell physiology · 2024Article
- Effect of knocking out mouseAmerican journal of physiology. Gastrointestinal and liver physiology · 2024Article
- Characterisation of Indian gut microbiome for B-vitamin production and its comparison with Chinese cohort.The British journal of nutrition · 2024Article
- Bacterial lipopolysaccharide inhibits colonic carrier-mediated uptake of thiamin pyrophosphate: roles for TLR4 receptor and NF-κB/P38/JNK signaling pathway.American journal of physiology. Cell physiology · 2023Article
- The differential plasma and ruminal metabolic pathways and ruminal bacterial taxa associated with divergent residual body weight gain phenotype in crossbred beef steers.Translational animal science · 2023Article
- Tumor necrosis factor α impedes colonic thiamin pyrophosphate and free thiamin uptake: involvement of JNK/ERKAmerican journal of physiology. Cell physiology · 2022Article
- In Silico Genomic and Metabolic Atlas ofMicroorganisms · 2022Article
- Hypoxia inhibits colonic uptake of the microbiota-generated forms of vitamin B1 via HIF-1α-mediated transcriptional regulation of their transporters.The Journal of biological chemistry · 2022Article
- Review
- Fursultiamine Prevents Drug-Induced Ototoxicity by Reducing Accumulation of Reactive Oxygen Species in Mouse Cochlea.Antioxidants (Basel, Switzerland) · 2021Article
- What We Know So Far about the Metabolite-Mediated Microbiota-Intestinal Immunity Dialogue and How to Hear the Sound of This Crosstalk.Metabolites · 2021Review
- Developmental maturation of the colonic uptake process of the microbiota-generated thiamin pyrophosphate.American journal of physiology. Gastrointestinal and liver physiology · 2021Article
- Immunometabolism, Micronutrients, and Bariatric Surgery: The Use of Transcriptomics and Microbiota-Targeted Therapies.Mediators of inflammation · 2020Review
- Enterohemorrhagic Escherichia coli infection inhibits colonic thiamin pyrophosphate uptake via transcriptional mechanism.PloS one · 2019Article
- Molecular mechanisms involved in the adaptive regulation of the colonic thiamin pyrophosphate uptake process.American journal of physiology. Cell physiology · 2017Article
- The human colonic thiamine pyrophosphate transporter (hTPPT) is a glycoprotein and N-linked glycosylation is important for its function.Biochimica et biophysica acta · 2016Article
- Mechanism(S) Involved in the Colon-Specific Expression of the Thiamine Pyrophosphate (Tpp) Transporter.PloS one · 2016Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
All mammals require exogenous sources of thiamine (vitamin B1), as they lack the ability to synthesize the vitamin. These sources are dietary and bacterial (the latter is in reference to the vitamin, which is synthesized by the normal microflora of the large intestine). Bacterially generated thiamine exists in the free, as well as the pyrophosphorylated [thiamine pyrophosphate (TPP)], form. With no (or very little) phosphatase activity in the colon, we hypothesized that the bacterially generated TPP can also be taken up by colonocytes. To test this hypothesis, we examined [(3)H]TPP uptake in the human-derived, nontransformed colonic epithelial NCM460 cells and purified apical membrane vesicles isolated from the colon of human organ donors. Uptake of TPP by NCM460 cells occurred without metabolic alterations in the transported substrate and 1) was pH- and Na(+)-independent, but energy-dependent, 2) was saturable as a function of concentration (apparent K(m) = 0.157 ± 0.028 μM), 3) was highly specific for TPP and not affected by free thiamine (or its analogs) or by thiamine monophosphate and unrelated folate derivatives, 4) was adaptively regulated by extracellular substrate (TPP) level via what appears to be a transcriptionally mediated mechanism(s), and 5) appeared to be influenced by an intracellular Ca(2+)/calmodulin-mediated regulatory pathway. These findings suggest the involvement of a carrier-mediated mechanism for TPP uptake by colonic NCM460 cells, which was further confirmed by results from studies of native human colonic apical membrane vesicles. The results also suggest that the bacterially synthesized TPP in the large intestine is bioavailable and may contribute to overall body homeostasis of vitamin B1 and, especially, to the cellular nutrition of the local colonocytes.
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