ArticleTheriogenology2012
Regulation of oocyte mitochondrial DNA copy number by follicular fluid, EGF, and neuregulin 1 during in vitro maturation affects embryo development in pigs.
Article in Theriogenology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 51 citations in OpenAlex.
- Molecular mechanisms of folliculogenesis and oogenesis.Systems biology in reproductive medicine · 2026Review
- Analysis of Relative Telomere Length and mtDNA Content in Individual Oocytes and Embryos by Quantitative Real-Time PCR (qPCR).Methods in molecular biology (Clifton, N.J.) · 2026Article
- Unravelling High Nuclear Genomic Similarity and Mitochondria Linked Epigenetic Divergence in SCNT Derived Buffalo Clones via Long-Read Nanopore Genome Sequencing.International journal of molecular sciences · 2025Article
- Optimization of handmade cloning enhances pregnancy rates and live calf production in cattle.Scientific reports · 2025Article
- Cytokine-Supplemented Maturation Medium Enhances Cytoplasmic and Nuclear Maturation in Bovine Oocytes.Animals : an open access journal from MDPI · 2024Article
- Maternal and paternal obesity differentially reprogram the ovarian mitochondrial biogenesis of F1 female rats.Scientific reports · 2023Article
- Oocyte secreted factors control genes regulating FSH signaling and the maturation cascade in cumulus cells: the oocyte is not in a hurry.Journal of assisted reproduction and genetics · 2023Article
- The role of mtDNA in oocyte quality and embryo development.Molecular reproduction and development · 2023Review
- Mitochondrial DNA Deficiency and Supplementation inInternational journal of molecular sciences · 2023Article
- 2cChIP-seq and 2cMeDIP-seq: The Carrier-Assisted Methods for Epigenomic Profiling of Small Cell Numbers or Single Cells.International journal of molecular sciences · 2022Article
- Anti-Müllerian hormone independently affect mtDNA copy number in human granulosa cells.Journal of ovarian research · 2022Article
- Preovulatory serum estradiol concentration is positively associated with oocyte ATP and follicular fluid metabolite abundance in lactating beef cattle.Journal of animal science · 2022Article
- Genistein Up-Regulates the Expression of EGF and E-Cadherin in the Treatment of Senile Vaginitis.Molecules (Basel, Switzerland) · 2022Article
- Concurrent Measurement of Mitochondrial DNA Copy Number and ATP Concentration in Single Bovine Oocytes.Methods and protocols · 2021Article
- The Role of Mitochondria in Oocyte Maturation.Cells · 2021Review
- Prediction of major microRNAs in follicular fluid regulating porcine oocyte development.Journal of assisted reproduction and genetics · 2020Article
- Article
- Mitochondria in oocyte aging: current understanding.Facts, views & vision in ObGyn · 2017Review
- Age-associated events in bovine oocytes and possible countermeasures.Reproductive medicine and biology · 2016Review
- Glycine supplementation in vitro enhances porcine preimplantation embryo cell number and decreases apoptosis but does not lead to live births.Molecular reproduction and development · 2016Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 2 countries.
Funding
Abstract
Little is known about mitochondrial DNA (mtDNA) replication during oocyte maturation and its regulation by extracellular factors. The present study determined the effects of supplementation of maturation medium with porcine follicular fluid (pFF; 0, 10%, 20%, and 30%) on mtDNA copy number and oocyte maturation in experiment 1; the effects on epidermal growth factor (EGF; 10 ng/mL), neuregulin 1 (NRG1; 20 ng/mL), and NRG1 + insulin-like growth factor 1 (IGF1; 100 ng/mL + NRG1 20 ng/mL), on mtDNA copy number, oocyte maturation, and embryo development after parthenogenic activation in experiment 2; and effects on embryo development after in vitro fertilization in experiment 3. Overall, mtDNA copy number increased from germinal vesicle (GV) to metaphase II (MII) stage oocytes after in vitro maturation (GV: 167 634.6 ± 20 740.4 vs. MII: 275 131.9 ± 9 758.4 in experiment 1; P < 0.05; GV: 185 004.7 ± 20 089.3 vs. MII: 239 392.8 ± 10 345.3 in experiment 2; P < 0.05; Least Squares Means ± SEM). Supplementation of IVM medium with pFF inhibited mtDNA replication (266 789.9 ± 11 790.4 vs. 318 510.1 ± 20 377.4; P < 0.05) and oocyte meiotic maturation (67.3 ± 0.7% vs. 73.2 ± 1.2%, for the pFF supplemented and zero pFF control, respectively; P < 0.01). Compared with the control, addition of growth factors enhanced oocyte maturation. Furthermore, supplementation of NRG1 stimulated mitochondrial replication, increased mtDNA copies in MII oocytes than in GV oocytes, and increased percentage of blastocysts in both parthenogenetic and in vitro fertilized embryos. In this study, mitochondrial biogenesis in oocytes was stimulated during in vitro maturation. Oocyte mtDNA copy number was associated with developmental competence. Supplementation of maturation medium with NRG1 increased mtDNA copy number, and thus provides a means to improve oocyte quality and developmental competence in pigs.
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