ArticleMolecular microbiology2012
Pichia pastoris 14-3-3 regulates transcriptional activity of the methanol inducible transcription factor Mxr1 by direct interaction.
Article in Molecular microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 49 citations in OpenAlex.
- Transcriptome analysis reveals methanol metabolism variations for the growth damage caused by overexpression of chimeric transactivators inSynthetic and systems biotechnology · 2025Article
- Regulation of Caenorhabditis elegans HLH-30 subcellular localization dynamics: Evidence for a redox-dependent mechanism.Free radical biology & medicine · 2024Article
- De novo biosynthesis of 2'-fucosyllactose in engineered Pichia pastoris.Biotechnology letters · 2023Article
- Enhancing xylanase expression of Komagataella phaffii induced by formate through Mit1 co-expression.Bioprocess and biosystems engineering · 2022Article
- Article
- Engineering of Promoters for Gene Expression in Pichia pastoris.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Regulation of Peroxisome Homeostasis by Post-Translational Modification in the Methylotrophic YeastFrontiers in cell and developmental biology · 2022Review
- Transcriptome Analysis Unveils the Effects of Proline on Gene Expression in the YeastMicroorganisms · 2021Article
- What makes Komagataella phaffii non-conventional?FEMS yeast research · 2021Review
- Transcriptomic Analysis ofFrontiers in microbiology · 2020Article
- Engineering the expression system for Komagataella phaffii (Pichia pastoris): an attempt to develop a methanol-free expression system.FEMS yeast research · 2019Article
- Ethanol represses the expression of methanol-inducible genes via acetyl-CoA synthesis in the yeast Komagataella phaffii.Scientific reports · 2018Article
- Presence of protein production enhancers results in significantly higher methanol-induced protein production in Pichia pastoris.Microbial cell factories · 2018Article
- Increased dosage of AOX1 promoter-regulated expression cassettes leads to transcription attenuation of the methanol metabolism in Pichia pastoris.Scientific reports · 2017Article
- Methanol Expression Regulator 1 (Mxr1p) Is Essential for the Utilization of Amino Acids as the Sole Source of Carbon by the Methylotrophic Yeast, Pichia pastoris.The Journal of biological chemistry · 2016Article
- Integration event induced changes in recombinant protein productivity in Pichia pastoris discovered by whole genome sequencing and derived vector optimization.Microbial cell factories · 2016Article
- Mit1 Transcription Factor Mediates Methanol Signaling and Regulates the Alcohol Oxidase 1 (AOX1) Promoter in Pichia pastoris.The Journal of biological chemistry · 2016Article
- Regulation of Acetate Metabolism and Acetyl Co-a Synthetase 1 (ACS1) Expression by Methanol Expression Regulator 1 (Mxr1p) in the Methylotrophic Yeast Pichia pastoris.The Journal of biological chemistry · 2016Article
- Article
- Yeast 14-3-3 protein functions as a comodulator of transcription by inhibiting coactivator functions.The Journal of biological chemistry · 2014Article
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4 authors at 1 institution in 1 country.
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Abstract
The zinc-finger transcription factor, Mxr1 activates methanol utilization and peroxisome biogenesis genes in the methylotrophic yeast, Pichia pastoris. Expression of Mxr1-dependent genes is regulated in response to various carbon sources by an unknown mechanism. We show here that this mechanism involves the highly conserved 14-3-3 proteins. 14-3-3 proteins participate in many biological processes in different eukaryotes. We have characterized a putative 14-3-3 binding region at Mxr1 residues 212-225 and mapped the major activation domain of Mxr1 to residues 246-280, and showed that phenylalanine residues in this region are critical for its function. Furthermore, we report that a unique and previously uncharacterized 14-3-3 family protein in P. pastoris complements Saccharomyces cerevisiae 14-3-3 functions and interacts with Mxr1 through its 14-3-3 binding region via phosphorylation of Ser215 in a carbon source-dependent manner. Indeed, our in vivo results suggest a carbon source-dependent regulation of expression of Mxr1-activated genes by 14-3-3 in P. pastoris. Interestingly, we observed 14-3-3-independent binding of Mxr1 to the promoters, suggesting a post-DNA binding function of 14-3-3 in regulating transcription. We provide the first molecular explanation of carbon source-mediated regulation of Mxr1 activity, whose mechanism involves a post-DNA binding role of 14-3-3.
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