ArticleAnalytical chemistry2012
Selection strategy to generate aptamer pairs that bind to distinct sites on protein targets.
Article in Analytical chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- The Role of Surfactants in Stabilizing Fluorescence Anisotropy for Protein-Aptamer Binding Affinity Measurements.Biosensors · 2025Article
- Screening a DNA Aptamer Specifically Targeting Integrin β3 and Partially Inhibiting Tumor Cell Migration.Analytical chemistry · 2023Article
- Aptamers Targeting Membrane Proteins for Sensor and Diagnostic Applications.Molecules (Basel, Switzerland) · 2023Review
- [Efficient screening for 8-oxoguanine DNA glycosylase binding aptamers via capillary electrophoresis].Se pu = Chinese journal of chromatography · 2021Article
- Review
- RNA Aptamers Targeting Integrin α5β1 as Probes for Cyto- and Histofluorescence in Glioblastoma.Molecular therapy. Nucleic acids · 2019Article
- Updates on Aptamer Research.International journal of molecular sciences · 2019Review
- Target-switched triplex nanotweezer and synergic fluorophore translocation for highly selective melamine assay.Mikrochimica acta · 2018Article
- Review
- Selection of DNA aptamers with two modified bases.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Aptamer-based sandwich-type biosensors.Journal of biological engineering · 2017Review
- The expanding world of DNA and RNA.Current opinion in chemical biology · 2016Review
- Characterization of the Neisseria gonorrhoeae Iron and Fur Regulatory Network.Journal of bacteriology · 2016Article
- Methods for Improving Aptamer Binding Affinity.Molecules (Basel, Switzerland) · 2016Review
- Generation of Aptamers with an Expanded Chemical Repertoire.Molecules (Basel, Switzerland) · 2015Review
- Identifying high-affinity aptamer ligands with defined cross-reactivity using high-throughput guided systematic evolution of ligands by exponential enrichment.Nucleic acids research · 2015Article
- Array-based discovery of aptamer pairs.Analytical chemistry · 2015Article
- RAID3--An interleukin-6 receptor-binding aptamer with post-selective modification-resistant affinity.RNA biology · 2015Article
- Nucleic Acid Ligands With Protein-like Side Chains: Modified Aptamers and Their Use as Diagnostic and Therapeutic Agents.Molecular therapy. Nucleic acids · 2014Review
- New Technologies Provide Quantum Changes in the Scale, Speed, and Success of SELEX Methods and Aptamer Characterization.Molecular therapy. Nucleic acids · 2014Article
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Authors and funding
10 authors.
Funding
Abstract
Many analytical techniques benefit greatly from the use of affinity reagent pairs, wherein each reagent recognizes a discrete binding site on a target. For example, antibody pairs have been widely used to dramatically increase the specificity of enzyme linked immunosorbent assays (ELISA). Nucleic acid-based aptamers offer many advantageous features relative to protein-based affinity reagents, including well-established chemical synthesis, thermostability, and low production cost. However, the generation of suitable aptamer pairs has posed a significant challenge, and few such pairs have been reported to date. To address this important challenge, we present multivalent aptamer isolation systematic evolution of ligands by exponential enrichment (MAI-SELEX), a technique designed for the efficient selection of aptamer pairs. In contrast to conventional selection methods, our method utilizes two selection modules to generate separate aptamer pools that recognize distinct binding sites on a single target. Using MAI-SELEX, we have isolated two groups of 2'-fluoro-modified RNA aptamers that specifically recognize the αV or β3 subunits of integrin αVβ3. These aptamers exhibit low nanomolar affinities for their targets, with minimal cross-reactivity to other closely related integrin homologues. Moreover, we show that these aptamer pairs do not interfere with each other's binding and effectively detect the target even in complex mixtures such as undiluted serum.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.