ArticleTissue engineering. Part C, Methods2012
Noninvasive, quantitative, spatiotemporal characterization of mineralization in three-dimensional collagen hydrogels using high-resolution spectral ultrasound imaging.
Article in Tissue engineering. Part C, Methods, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Methacrylate-Modified Gold Nanoparticles Enable Non-Invasive Monitoring of Photocrosslinked Hydrogel Scaffolds.Advanced nanobiomed research · 2022Article
- Therapeutic Ultrasound Effects on Human Induced Pluripotent Stem Cell Cardiomyocytes Measured Optically and with Spectral Ultrasound.Ultrasound in medicine & biology · 2022Article
- High Frequency Spectral Ultrasound Imaging Detects Early Heterotopic Ossification in Rodents.Stem cells and development · 2021Article
- A novel quantitative and reference-free ultrasound analysis to discriminate different concentrations of bone mineral content.Scientific reports · 2021Article
- Photoacoustic Imaging in Tissue Engineering and Regenerative Medicine.Tissue engineering. Part B, Reviews · 2020Review
- High Frequency Spectral Ultrasound Imaging to Detect Metastasis in Implanted Biomaterial Scaffolds.Annals of biomedical engineering · 2020Article
- Spatiotemporal delivery of basic fibroblast growth factor to directly and simultaneously attenuate cardiac fibrosis and promote cardiac tissue vascularization following myocardial infarction.Journal of controlled release : official journal of the Controlled Release Society · 2019Article
- Multimodal Label-Free Imaging for Detecting Maturation of Engineered Osteogenic Grafts.ACS biomaterials science & engineering · 2019Article
- Dispersible hydrogel force sensors reveal patterns of solid mechanical stress in multicellular spheroid cultures.Nature communications · 2019Article
- Biopolymeric In Situ Hydrogels for Tissue Engineering and Bioimaging Applications.Tissue engineering and regenerative medicine · 2018Review
- Multimode ultrasound viscoelastography for three-dimensional interrogation of microscale mechanical properties in heterogeneous biomaterials.Biomaterials · 2018Article
- Imaging Biomaterial-Tissue Interactions.Trends in biotechnology · 2018Review
- Article
- An Injectable Oxygen Release System to Augment Cell Survival and Promote Cardiac Repair Following Myocardial Infarction.Scientific reports · 2018Article
- Ultrasound Imaging Techniques for Spatiotemporal Characterization of Composition, Microstructure, and Mechanical Properties in Tissue Engineering.Tissue engineering. Part B, Reviews · 2016Review
- Microscale characterization of the viscoelastic properties of hydrogel biomaterials using dual-mode ultrasound elastography.Biomaterials · 2016Article
- Quantitative Ultrasound for Nondestructive Characterization of Engineered Tissues and Biomaterials.Annals of biomedical engineering · 2016Review
- Noninvasive Quantitative Imaging of Collagen Microstructure in Three-Dimensional Hydrogels Using High-Frequency Ultrasound.Tissue engineering. Part C, Methods · 2015Article
- Dual-phase osteogenic and vasculogenic engineered tissue for bone formation.Tissue engineering. Part A · 2015Article
- Imaging strategies for tissue engineering applications.Tissue engineering. Part B, Reviews · 2015Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
As tissue engineering products move toward the clinic, nondestructive methods to monitor their development and ensure quality are needed. In this study, high-resolution spectral ultrasound imaging (SUSI) was used to noninvasively characterize mineral content in collagen hydrogels. SUSI was used to generate three-dimensional (3D) grayscale (GS) images of construct morphology with submillimeter resolution. Spectral analysis of the backscattered radio frequency (RF) ultrasound signals was used to determine the midband fit (MBF) and slope of the linearized RF spectrum. These parameters are operator and instrument independent, and were used to characterize the spatial distribution of mineral in constructs supplemented with hydroxyapatite particles. GS and MBF correlated closely with mineral content, while slope was not dependent on concentration. SUSI also was used to monitor mineralization of collagen constructs by immersion in simulated body fluid (SBF) over 21 days. The construct surface was mineralized before the interior, and there was a dose-dependent effect of SBF concentration on degree of mineralization and deposited particle size. MBF density was closely correlated with the amount of calcium deposited. These data demonstrate that SUSI has utility as a noninvasive imaging method for quantitative analysis of mineralization in 3D protein constructs. Such techniques may assist the development of engineered orthopedic tissues.
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