ArticlePloS one2012
Expression of an epitope-tagged virulence protein in Rickettsia parkeri using transposon insertion.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Transformation and allelic exchange inmBio · 2026Article
- Transformation and allelic exchange inbioRxiv : the preprint server for biology · 2026Article
- An expanded genetic toolkit for inducible expression and targeted gene silencing inJournal of bacteriology · 2024Article
- Cell-selective proteomics reveal novel effectors secreted by an obligate intracellular bacterial pathogen.Nature communications · 2024Article
- The Function of Termicin fromInsects · 2024Article
- An expanded genetic toolkit for inducible expression and targeted gene silencing inbioRxiv : the preprint server for biology · 2024Article
- PathogenicJournal of bacteriology · 2024Review
- Recent advances in genetic systems in obligate intracellular human-pathogenic bacteria.Frontiers in cellular and infection microbiology · 2023Review
- Evasion of autophagy mediated by Rickettsia surface protein OmpB is critical for virulence.Nature microbiology · 2019Article
- A streamlined method for transposon mutagenesis of Rickettsia parkeri yields numerous mutations that impact infection.PloS one · 2018Article
- Electrotransformation and Clonal Isolation of Rickettsia Species.Current protocols in microbiology · 2015Article
- Motility characteristics are altered for Rickettsia bellii transformed to overexpress a heterologous rickA gene.Applied and environmental microbiology · 2014Article
- Genetic systems for studying obligate intracellular pathogens: an update.Current opinion in microbiology · 2014Review
- Rickettsia actin-based motility occurs in distinct phases mediated by different actin nucleators.Current biology : CB · 2014Article
- Breaking in and grabbing a meal: Anaplasma phagocytophilum cellular invasion, nutrient acquisition, and promising tools for their study.Microbes and infection · 2013Review
- Recent molecular insights into rickettsial pathogenesis and immunity.Future microbiology · 2013Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Despite recent advances in our ability to genetically manipulate Rickettsia, little has been done to employ genetic tools to study the expression and localization of Rickettsia virulence proteins. Using a mariner-based Himar1 transposition system, we expressed an epitope-tagged variant of the actin polymerizing protein RickA under the control of its native promoter in Rickettsia parkeri, allowing the detection of RickA using commercially-available antibodies. Native RickA and epitope-tagged RickA exhibited similar levels of expression and were specifically localized to bacteria. To further facilitate protein expression in Rickettsia, we also developed a plasmid for Rickettsia insertion and expression (pRIE), containing a variant Himar1 transposon with enhanced flexibility for gene insertion, and used it to generate R. parkeri strains expressing diverse fluorescent proteins. Expression of epitope-tagged proteins in Rickettsia will expand our ability to assess the regulation and function of important virulence factors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.