ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2012
In vivo visualization of delta opioid receptors upon physiological activation uncovers a distinct internalization profile.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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Who cites it
22 citing papers in PubMed, 43 citations in OpenAlex.
- Extrinsic and intrinsic control of striatal cholinergic interneuron activity.Frontiers in molecular neuroscience · 2025Review
- Mapping Astrocytic and Neuronal μ-opioid Receptor Expression in Various Brain Regions Using MOR-mCherry Reporter Mouse.Experimental neurobiology · 2023Article
- Receptor expression and signaling properties in the brain, and structural ligand motifs that contribute to delta opioid receptor agonist-induced seizures.Neuropharmacology · 2023Review
- Effects of long-term postgastric infusion of casein or glutamic acid on small intestinal starch digestion and energy balance in cattle.Journal of animal science · 2023Article
- Article
- Heteromerization of Endogenous Mu and Delta Opioid Receptors Induces Ligand-Selective Co-Targeting to Lysosomes.Molecules (Basel, Switzerland) · 2020Article
- Mapping GPR88-Venus illuminates a novel role for GPR88 in sensory processing.Brain structure & function · 2018Article
- Strategy for Identification of Phosphorylation Levels of Low Abundance Proteins in Vivo for Which Antibodies Are not Available.Journal of cardiovascular development and disease · 2017Article
- The delta opioid receptor tool box.Neuroscience · 2016Review
- Sex differences in subcellular distribution of delta opioid receptors in the rat hippocampus in response to acute and chronic stress.Neurobiology of stress · 2016Article
- Molecular Pharmacology of δ-Opioid Receptors.Pharmacological reviews · 2016Review
- Impact of chronic morphine on delta opioid receptor-expressing neurons in the mouse hippocampus.Neuroscience · 2016Article
- A mu-delta opioid receptor brain atlas reveals neuronal co-occurrence in subcortical networks.Brain structure & function · 2015Article
- In vivo techniques to investigate the internalization profile of opioid receptors.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Anti-nociception mediated by a κ opioid receptor agonist is blocked by a δ receptor agonist.British journal of pharmacology · 2015Article
- The role of δ-opioid receptors in learning and memory underlying the development of addiction.British journal of pharmacology · 2015Review
- Fluorescent knock-in mice to decipher the physiopathological role of G protein-coupled receptors.Frontiers in pharmacology · 2014Review
- Learning-related translocation of δ-opioid receptors on ventral striatal cholinergic interneurons mediates choice between goal-directed actions.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Delta-opioid receptors mediate unique plasticity onto parvalbumin-expressing interneurons in area CA2 of the hippocampus.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- The multiple facets of opioid receptor function: implications for addiction.Current opinion in neurobiology · 2013Review
Corrections and comments
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Authors and funding
14 authors at 5 institutions in 2 countries.
Funding
Abstract
G-protein-coupled receptors (GPCRs) mediate numerous physiological functions and represent prime therapeutic targets. Receptor trafficking upon agonist stimulation is critical for GPCR function, but examining this process in vivo remains a true challenge. Using knock-in mice expressing functional fluorescent delta opioid receptors under the control of the endogenous promoter, we visualized in vivo internalization of this native GPCR upon physiological stimulation. We developed a paradigm in which animals were made dependent on morphine in a drug-paired context. When re-exposed to this context in a drug-free state, mice showed context-dependent withdrawal signs and activation of the hippocampus. Receptor internalization was transiently detected in a subset of CA1 neurons, uncovering regionally restricted opioid peptide release. Importantly, a pool of surface receptors always remained, which contrasts with the in vivo profile previously established for exogenous drug-induced internalization. Therefore, a distinct response is observed at the receptor level upon a physiological or pharmacological stimulation. Altogether, direct in vivo GPCR visualization enables mapping receptor stimulation promoted by a behavioral challenge and represents a powerful approach to study endogenous GPCR physiology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.