ArticleCell death & disease2012
In vitro differentiation of mouse embryonic stem cells into inner ear hair cell-like cells using stromal cell conditioned medium.
Article in Cell death & disease, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Advances in Microfluidic Cochlea-On-A-Chip.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Modern In Vitro Techniques for Modeling Hearing Loss.Bioengineering (Basel, Switzerland) · 2024Review
- Modelling inner ear development and disease using pluripotent stem cells - a pathway to new therapeutic strategies.Disease models & mechanisms · 2022Review
- In vitro and in vivo models: What have we learnt about inner ear regeneration and treatment for hearing loss?Molecular and cellular neurosciences · 2022Review
- Deafness-in-a-dish: modeling hereditary deafness with inner ear organoids.Human genetics · 2022Review
- Inner Ear Drug Delivery for Sensorineural Hearing Loss: Current Challenges and Opportunities.Frontiers in neuroscience · 2022Review
- Differentiation of embryonic stem cells into a putative hair cell-progenitor cells via co-culture with HEI-OC1 cells.Scientific reports · 2021Article
- Stem cell-based approaches: Possible route to hearing restoration?World journal of stem cells · 2020Review
- Progress in Modeling and Targeting Inner Ear Disorders with Pluripotent Stem Cells.Stem cell reports · 2020Review
- Differentiation of embryonic stem cells into inner ear vestibular hair cells using vestibular cell derived-conditioned medium.Biochemistry and biophysics reports · 2019Article
- Pluripotent stem cell-derived cochlear cells: a challenge in constant progress.Cellular and molecular life sciences : CMLS · 2019Review
- Generating inner ear organoids containing putative cochlear hair cells from human pluripotent stem cells.Cell death & disease · 2018Article
- Induction of lung-like cells from mouse embryonic stem cells by decellularized lung matrix.Biochemistry and biophysics reports · 2018Article
- Potential of Gene and Cell Therapy for Inner Ear Hair Cells.BioMed research international · 2018Review
- Modeling human early otic sensory cell development with induced pluripotent stem cells.PloS one · 2018Article
- Gene array analysis of neural crest cells identifies transcription factors necessary for direct conversion of embryonic fibroblasts into neural crest cells.Biology open · 2016Article
- Sensational placodes: neurogenesis in the otic and olfactory systems.Developmental biology · 2014Review
- 3D mouse embryonic stem cell culture for generating inner ear organoids.Nature protocols · 2014Article
- miR-24 affects hair follicle morphogenesis targeting Tcf-3.Cell death & disease · 2013Article
- Article
Corrections and comments
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Authors and funding
4 authors.
Funding
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Abstract
Hearing loss is mainly caused by loss of sensory hair cells (HCs) in the organ of Corti or cochlea. Although embryonic stem (ES) cells are a promising source for cell therapy, little is known about the efficient generation of HC-like cells from ES cells. In the present study, we developed a single-medium culture method for growing embryoid bodies (EBs), in which conditioned medium (CM) from cultures of ST2 stromal cells (ST2-CM) was used for 14-day cultures of 4-day EBs. At the end of the 14-day cultures, up to 20% of the cells in EB outgrowths expressed HC-related markers, including Math1 (also known as Atoh1), myosin6, myosin7a, calretinin, α9AchR and Brn3c (also known as Pou4f3), and also showed formation of stereocilia-like structures. Further, we found that these cells were incorporated into the developing inner ear after transplantation into chick embryos. The present inner ear HC induction method using ST2-CM (HIST2 method) is quite simple and highly efficient to obtain ES-derived HC-like cells with a relatively short cultivation time.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.