ArticleThe Journal of biological chemistry2012
Disintegration of nascent replication bubbles during thymine starvation triggers RecA- and RecBCD-dependent replication origin destruction.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Article
- RecBCD-dependent post-UV replication restart inProceedings of the National Academy of Sciences of the United States of America · 2026Article
- UV induces codirectional replication-transcription conflicts and an alternative DnaA-dependent replication origin in the rnhAB mutants of Escherichiacoli.Nucleic acids research · 2025Article
- Defects in the central metabolism prevent thymineless death in Escherichia coli, while still allowing significant protein synthesis.Genetics · 2024Article
- Thymine-starvation-induced chromosomal fragmentation is not required for thymineless death in Escherichia coli.Molecular microbiology · 2022Article
- Oxidative Damage Blocks Thymineless Death and Trimethoprim Poisoning in Escherichia coli.Journal of bacteriology · 2022Article
- Electron Microscopy Reveals Unexpected Cytoplasm and Envelope Changes during Thymineless Death in Escherichia coli.Journal of bacteriology · 2021Article
- Exopolysaccharide defects cause hyper-thymineless death inProceedings of the National Academy of Sciences of the United States of America · 2020Article
- The Roles of Bacterial DNA Double-Strand Break Repair Proteins in Chromosomal DNA Replication.FEMS microbiology reviews · 2020Review
- Thymineless Death inJournal of bacteriology · 2019Article
- Sources of thymidine and analogs fueling futile damage-repair cycles and ss-gap accumulation during thymine starvation in Escherichia coli.DNA repair · 2019Article
- Near-continuously synthesized leading strands inProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Incomplete base excision repair contributes to cell death from antibiotics and other stresses.DNA repair · 2018Review
- Broken replication forks trigger heritable DNA breaks in the terminus of a circular chromosome.PLoS genetics · 2018Article
- A radical way to die.Nature microbiology · 2017Article
- Contribution of reactive oxygen species to thymineless death in Escherichia coli.Nature microbiology · 2017Article
- Degradation of RNA during lysis of Escherichia coli cells in agarose plugs breaks the chromosome.PloS one · 2017Article
- Transcriptome Analysis of Escherichia coli during dGTP Starvation.Journal of bacteriology · 2016Article
- Static and Dynamic Factors Limit Chromosomal Replication Complexity in Escherichia coli, Avoiding Dangers of Runaway Overreplication.Genetics · 2016Article
- A DinB Ortholog Enables Mycobacterial Growth under dTTP-Limiting Conditions Induced by the Expression of a Mycobacteriophage-Derived Ribonucleotide Reductase Gene.Journal of bacteriology · 2016Article
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2 authors.
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Abstract
Thymineless death strikes cells unable to synthesize DNA precursor dTTP, with the nature of chromosomal damage still unclear. Thymine starvation stalls replication forks, whereas accumulating evidence indicates the replication origin is also affected. Using a novel DNA labeling technique, here we show that replication slowly continues in thymine-starved cells, but the newly synthesized DNA becomes fragmented and degraded. This degradation apparently releases enough thymine to sustain initiation of new replication bubbles from the chromosomal origin, which destabilizes the origin in a RecA-dependent manner. Marker frequency analysis with gene arrays 1) reveals destruction of the origin-centered chromosomal segment in RecA(+) cells; 2) confirms origin accumulation in the recA mutants; and 3) identifies the sites around the origin where destruction initiates in the recBCD mutants. We propose that thymineless cells convert persistent single-strand gaps behind replication forks into double-strand breaks, using the released thymine for new initiations, whereas subsequent disintegration of small replication bubbles causes replication origin destruction.
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