ArticleProteome science2012
Proteins differentially expressed in elicited cell suspension culture of Podophyllum hexandrum with enhanced podophyllotoxin content.
Article in Proteome science, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 59 citations in OpenAlex.
- Review
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- Plant stress and proteomics in medicinal plants.Frontiers in plant science · 2025Review
- Article
- The Current Developments in Medicinal Plant Genomics Enabled the Diversification of Secondary Metabolites' Biosynthesis.International journal of molecular sciences · 2022Review
- Stimulation of Lignan Production inMolecules (Basel, Switzerland) · 2022Article
- In Vitro Response ofCells · 2021Article
- Improved production of dibenzocyclooctadiene lignans in the elicited microshoot cultures of Schisandra chinensis (Chinese magnolia vine).Applied microbiology and biotechnology · 2018Article
- Methyl Jasmonate Regulates Podophyllotoxin Accumulation inFrontiers in plant science · 2017Article
- Transcriptome-wide identification and characterization of CAD isoforms specific for podophyllotoxin biosynthesis from Podophyllum hexandrum.Plant molecular biology · 2016Article
- Expression analysis of biosynthetic pathway genes vis-à-vis podophyllotoxin content in Podophyllum hexandrum Royle.Protoplasma · 2015Article
- Essences in metabolic engineering of lignan biosynthesis.Metabolites · 2015Review
- The role of proteomics in progressing insights into plant secondary metabolism.Frontiers in plant science · 2015Review
- Transcriptome sequencing of rhizome tissue of Sinopodophyllum hexandrum at two temperatures.BMC genomics · 2014Article
- Mass spectrometric analysis of differentially expressed proteins in an endangered medicinal herb, Picrorhiza kurroa.BioMed research international · 2014Article
- De novo transcriptome analysis using 454 pyrosequencing of the Himalayan Mayapple, Podophyllum hexandrum.BMC genomics · 2013Article
- Plant proteomics in India and Nepal: current status and challenges ahead.Physiology and molecular biology of plants : an international journal of functional plant biology · 2013Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
unlabelled
backgroundPodophyllotoxin (PTOX), the precursor for semi-synthesis of cancer therapeutics like etoposide, teniposide and etophos, is primarily obtained from an endangered medicinal herb, Podophyllum hexandrum Royle. PTOX, a lignan is biosynthetically derived from the phenylpropanoid pathway. The aim of this study is to investigate changes in the P. hexandrum cell proteome potentially related to PTOX accumulation in response to methyl jasmonate (MeJA) elicitation. High-resolution two-dimensional gel electrophoresis (2-DE) followed by colloidal Coomassie staining and mass spectrometric analysis was used to detect statistically significant changes in cell's proteome.
resultThe HPLC analysis showed approximately 7-8 fold change in accumulation of PTOX, in the 12day old cell suspension culture (i.e. after 9days of elicitation) elicited with 100 μM MeJA as compared to the control. Using 2-DE a total of 233 spots was detected, out of which 105 spots were identified by MALDI TOF-TOF MS/MS. Data were subjected to functional annotation from a biological point of view through KEGG. The phenylpropanoid and monolignol pathway enzymes were identified, amongst these, chalcone synthase, polyphenol oxidase, caffeoyl CoA 3-O-methyltransferase, S-adenosyl-L-methionine-dependent methyltransferases, caffeic acid-O-methyl transferase etc. are noted as important. The relation of other differentially accumulated proteins with varied effects caused by elicitors on P. hexandrum cells namely stress and defense related protein, transcription and DNA replication and signaling are also discussed.
conclusionsElicitor-induced PTOX accumulation in P. hexandrum cell cultures provides a responsive model system to profile modulations in proteins related to phenylpropanoid/monolignol biosynthesis and other defense responses. Present findings form a baseline for future investigation on a non-sequenced medicinal herb P. hexandrum at molecular level.
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Registered trials
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