ArticleDiabetologia2012
Differentiating neural crest stem cells induce proliferation of cultured rodent islet beta cells.
Article in Diabetologia, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 32 citations in OpenAlex.
- Boundary cap neural crest stem cells exhibit remarkable resilience to environmental stressors associated with International Space Station mission.NPJ microgravity · 2026Article
- Microgravity Effect on Pancreatic Islets.Cells · 2024Article
- Effects of microgravity on neural crest stem cells.Frontiers in neuroscience · 2024Article
- Towards 3D Bioprinted Spinal Cord Organoids.International journal of molecular sciences · 2022Article
- Empty mesoporous silica particles significantly delay disease progression and extend survival in a mouse model of ALS.Scientific reports · 2020Article
- Characterization of neural crest-derived stem cells isolated from human bone marrow for improvement of transplanted islet function.Upsala journal of medical sciences · 2019Article
- Neural crest stem cells protect spinal cord neurons from excitotoxic damage and inhibit glial activation by secretion of brain-derived neurotrophic factor.Cell and tissue research · 2018Article
- Boundary Cap Neural Crest Stem Cells Promote Survival of Mutant SOD1 Motor Neurons.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2017Article
- Neural cells play an inhibitory role in pancreatic differentiation of pluripotent stem cells.Genes to cells : devoted to molecular & cellular mechanisms · 2015Article
- Review
- Generation of β cells from human pluripotent stem cells: are we there yet?Annals of the New York Academy of Sciences · 2014Review
- Human β-cell regeneration: progress, hurdles, and controversy.Current opinion in endocrinology, diabetes, and obesity · 2014Review
- Review
- Article
- Altered pancreatic islet function and morphology in mice lacking the Beta-cell surface protein neuroligin-2.PloS one · 2013Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aims/hypothesisEfficient stimulation of cycling activity in cultured beta cells would allow the design of new strategies for cell therapy in diabetes. Neural crest stem cells (NCSCs) play a role in beta cell development and maturation and increase the beta cell number in co-transplants. The mechanism behind NCSC-induced beta cell proliferation and the functional capacity of the new beta cells is not known.
methodsWe developed a new in vitro co-culture system that enables the dissection of the elements that control the cellular interactions that lead to NCSC-dependent increase in islet beta cells.
resultsMouse NCSCs were cultured in vitro, first in medium that stimulated their proliferation, then under conditions that supported their differentiation. When mouse islet cells were cultured together with the NCSCs, more than 35% of the beta cells showed cycle activity. This labelling index is more than tenfold higher than control islets cultured without NCSCs. Beta cells that proliferated under these culture conditions were fully glucose responsive in terms of insulin secretion. NCSCs also induced beta cell proliferation in islets isolated from 1-year-old mice, but not in dissociated islet cells isolated from human donor pancreas tissue. To stimulate beta cell proliferation, NCSCs need to be in intimate contact with the beta cells. CONCLUSIONS/
interpretationCulture of islet cells in contact with NCSCs induces highly efficient beta cell proliferation. The reported culture system is an excellent platform for further dissection of the minimal set of factors needed to drive this process and explore its potential for translation to diabetes therapy.
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Registered trials
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