ArticleMenopause (New York, N.Y.)2012
Effects of long-term treatment with estrogen and medroxyprogesterone acetate on synapse number in the medial prefrontal cortex of aged female rats.
Article in Menopause (New York, N.Y.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 27 citations in OpenAlex.
- Menopause and neurological disorders: a roadmap for research.Nature reviews. Neurology · 2026Review
- Medroxyprogesterone opposes estradiol-induced renal damage in midlife ovariectomized Long Evans rats.Menopause (New York, N.Y.) · 2020Article
- The effects of early life stress on motivated behaviors: A role for gonadal hormones.Neuroscience and biobehavioral reviews · 2020Review
- Dopamine-induced interactions of female mouse hypothalamic proteins with progestin receptor-A in the absence of hormone.Journal of neuroendocrinology · 2020Article
- Applying a Women's Health Lens to the Study of the Aging Brain.Frontiers in human neuroscience · 2019Article
- Striatal transcriptome of a mouse model of ADHD reveals a pattern of synaptic remodeling.PloS one · 2018Article
- Synaptic number changes in the medial prefrontal cortex across adolescence in male and female rats: A role for pubertal onset.Synapse (New York, N.Y.) · 2016Article
- Differential mesocorticolimbic responses to palatable food in binge eating prone and binge eating resistant female rats.Physiology & behavior · 2015Article
- Factors influencing the cognitive and neural effects of hormone treatment during aging in a rodent model.Brain research · 2013Review
- Males, but not females, lose tyrosine hydroxylase fibers in the medial prefrontal cortex and are impaired on a delayed alternation task during aging.Behavioural brain research · 2013Article
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2 authors at 1 institution in 1 country.
Funding
Abstract
objectiveThe present study investigated the effects of long-term hormone treatment, including the most commonly prescribed progestin, medroxyprogesterone acetate, during aging on synaptophysin-labeled boutons, a marker of synapses, in the medial prefrontal cortex (mPFC) of rats.
methodsFemale Long Evans hooded rats were ovariectomized at middle age (12-13 mo) and were placed in one of four groups: no replacement (n = 5), 17β-estradiol alone (n = 6), estradiol and progesterone (n = 7), or estradiol and medroxyprogesterone acetate (n = 4). Estradiol was administered in the drinking water and progestogens were administered via subcutaneous pellets that were replaced every 90 days. After 7 months of hormone replacement, the animals were euthanized, and the brains were stained for synaptophysin, a membrane component of synaptic vesicles. The density of synaptophysin-labeled boutons was quantified in the mPFC using unbiased stereology and multiplied by the volume of the mPFC to obtain the total number.
resultsAnimals receiving estradiol and medroxyprogesterone acetate had significantly more synaptophysin-labeled boutons in the mPFC than did animals not receiving replacement (P < 0.03) and those receiving estradiol and progesterone (P < 0.02). In addition, there was a nonsignificant trend for animals receiving estradiol alone to have more synapses than those receiving estradiol and progesterone.
conclusionsThis study is the first to examine the effects of estradiol and medroxyprogesterone acetate during rat aging on cortical synaptic number. Estradiol with medroxyprogesterone acetate, but not progesterone, resulted in a greater number of synapses in the mPFC during aging than did no replacement.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.