ArticleNature communications2012
Microtubule and cortical forces determine platelet size during vascular platelet production.
Article in Nature communications, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
68 citing papers in PubMed.
- Recent Advances in Bacterial Separation and Enrichment from Blood for the Diagnosis of Bloodstream Infections.Sensors (Basel, Switzerland) · 2026Review
- Altered thrombopoiesis in spontaneously hypertensive rats.Scientific reports · 2025Article
- Evidence for a cytoplasmic proplatelet promoting factor that triggers platelet production.Haematologica · 2024Article
- Cell cycle-dependent centrosome clustering precedes proplatelet formation.Science advances · 2024Article
- Megakaryocyte-induced contraction of plasma clots: cellular mechanisms and structural mechanobiology.Blood · 2024Article
- MXRA7 is involved in megakaryocyte differentiation and platelet production.Blood science (Baltimore, Md.) · 2023Article
- Review
- Direct Cryo-ET observation of platelet deformation induced by SARS-CoV-2 spike protein.Nature communications · 2023Article
- Proceedings of the immune thrombocytopenia summit: new concepts in mechanisms, diagnosis, and management.Research and practice in thrombosis and haemostasis · 2023Review
- A prestressed intracellular biomechanical model for the platelet to capture the disc-to-sphere morphological change from resting to activated state.International journal of computational methods · 2022Article
- Direct Cryo-ET observation of platelet deformation induced by SARS-CoV-2 Spike protein.bioRxiv : the preprint server for biology · 2022Article
- Analysis of preplatelets and their barbell platelet derivatives by imaging flow cytometry.Blood advances · 2022Article
- The triple crown of platelet generation.Blood · 2022Article
- Models of Shear-Induced Platelet Activation and Numerical Implementation With Computational Fluid Dynamics Approaches.Journal of biomechanical engineering · 2022Article
- Of vascular defense, hemostasis, cancer, and platelet biology: an evolutionary perspective.Cancer metastasis reviews · 2022Review
- Expanding the genetic spectrum of TUBB1-related thrombocytopenia.Blood advances · 2021Article
- The high platelet counts as predictor for early foetal demise.Annals of medicine · 2021Article
- Article
- The Importance of Alpha-Actinin Proteins in Platelet Formation and Function, and Their Causative Role in Congenital Macrothrombocytopenia.International journal of molecular sciences · 2021Review
- Patterning Microtubule Network Organization Reshapes Cell-Like Compartments.ACS synthetic biology · 2021Article
8 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
Megakaryocytes release large preplatelet intermediates into the sinusoidal blood vessels. Preplatelets convert into barbell-shaped proplatelets in vitro to undergo repeated abscissions that yield circulating platelets. These observations predict the presence of circular-preplatelets and barbell-proplatelets in blood, and two fundamental questions in platelet biology are what are the forces that determine barbell-proplatelet formation, and how is the final platelet size established. Here we provide insights into the terminal mechanisms of platelet production. We quantify circular-preplatelets and barbell-proplatelets in human blood in high-resolution fluorescence images, using a laser scanning cytometry assay. We demonstrate that force constraints resulting from cortical microtubule band diameter and thickness determine barbell-proplatelet formation. Finally, we provide a mathematical model for the preplatelet to barbell conversion. We conclude that platelet size is limited by microtubule bundling, elastic bending, and actin-myosin-spectrin cortex forces.
Indexed as
Identifiers
22617292What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.