ArticleActa orthopaedica2012
PCR-hybridization after sonication improves diagnosis of implant-related infection.
Article in Acta orthopaedica, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 4 of them syntheses that pooled it.
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Who cites it
39 citing papers in PubMed, 4 syntheses or guidelines pooled it, 85 citations in OpenAlex.
- Diagnostic accuracy of sonication fluid cultures from prosthetic components in periprosthetic joint infection: an updated diagnostic meta-analysis.Journal of orthopaedic surgery and research · 2023Pooled it
- Meta-analysis of sonication prosthetic fluid PCR for diagnosing periprosthetic joint infection.PloS one · 2018Pooled it
- Meta-analysis of sonication fluid samples from prosthetic components for diagnosis of infection after total joint arthroplasty.Journal of clinical microbiology · 2014Pooled it
- PCR-based diagnosis of prosthetic joint infection.Journal of clinical microbiology · 2013Pooled it
- Disinfection of contaminated metal implants with an Er:YAG laser.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2020Trial
- Value of multiplex PCR for detection of antimicrobial resistance in samples retrieved from patients with orthopaedic infections.BMC microbiology · 2020Trial
- The boundary between microbial detection and implant-related infection: a structured narrative review of analytical credibility, biological persistence, host response, and clinical causality.Archives of orthopaedic and trauma surgery · 2026Review
- Denaturation methods for reusable magnetic biosensors.Lab on a chip · 2025Article
- Biofilm and the effect of sonication in a chronic Staphylococcus epidermidis orthopedic in vivo implant infection model.Journal of orthopaedic surgery and research · 2024Article
- Advances in the Microbiological Diagnosis of Prosthetic Joint Infections.Diagnostics (Basel, Switzerland) · 2023Review
- The Diagnostic Value of Blood Next-Generation Sequencing in Early Surgical Site Infection After Spine Surgery.International journal of general medicine · 2023Article
- An update about molecular biology techniques to detect orthopaedic implant-related infections.EFORT open reviews · 2021Review
- Advantages of 16S rRNA PCR for the diagnosis of prosthetic joint infection.Experimental and therapeutic medicine · 2020Article
- The Impact of EBM-Manufactured Ti6Al4V ELI Alloy Surface Modifications on Cytotoxicity toward Eukaryotic Cells and Microbial Biofilm Formation.Materials (Basel, Switzerland) · 2020Article
- Sonication Leads to Clinically Relevant Changes in Treatment of Periprosthetic Hip or Knee Joint Infection.Journal of bone and joint infection · 2020Article
- Diagnosis of peripheral bone and prosthetic joint infections: overview on the consensus documents by the EANM, EBJIS, and ESR (with ESCMID endorsement).European radiology · 2019Review
- Consensus document for the diagnosis of prosthetic joint infections: a joint paper by the EANM, EBJIS, and ESR (with ESCMID endorsement).European journal of nuclear medicine and molecular imaging · 2019Article
- High percentage of microbial colonization of osteosynthesis material in clinically unremarkable patients.MicrobiologyOpen · 2019Article
- Evaluation of the use of sonication of retrieved implants for the diagnosis of prosthetic joint infection in a routine setting.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2018Article
- Performance of automated multiplex PCR using sonication fluid for diagnosis of periprosthetic joint infection: a prospective cohort.Infection · 2017Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeWe wanted to improve the diagnosis of implant-related infection using molecular biological techniques after sonication.
methodsWe studied 258 retrieved implant components (185 prosthetic implants and 73 osteosynthesis implants) from 126 patients. 47 patients had a clinical diagnosis of infection (108 components) and 79 patients did not (150 components). The fluids from sonication of retrieved implants were tested in culture and were also analyzed using a modified commercial PCR kit for detection of Gram-positive and Gram-negative bacteria (GenoType BC; Hain Lifescience) after extraction of the DNA.
results38 of 47 patients with a clinical diagnosis of infection were also diagnosed as being infected using culture and/or PCR (35 by culture alone). Also, 24 patients of the 79 cases with no clinical diagnosis of infection were identified microbiologically as being infected (4 by culture, 16 by PCR, and 4 by both culture and PCR). Comparing culture and PCR, positive culture results were obtained in 28 of the 79 patients and positive PCR results were obtained in 35. There were 21 discordant results in patients who were originally clinically diagnosed as being infected and 28 discordant results in patients who had no clinical diagnosis of infection.
interpretationFor prosthetic joint infections and relative to culture, molecular detection can increase (by one tenth) the number of patients diagnosed as having an infection. Positive results from patients who have no clinical diagnosis of infection must be interpreted carefully.
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