ArticlePloS one2012
Accelerated and improved quantification of lymphocytic choriomeningitis virus (LCMV) titers by flow cytometry.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 21 citations in OpenAlex.
- Specific inflammatory stimuli that engage innate immune sensors induce novel CD103 expression profiles in macrophages.Frontiers in cellular and infection microbiology · 2025Article
- Limited extent and consequences of pancreatic SARS-CoV-2 infection.Cell reports · 2022Article
- Differential TLR7-mediated cytokine expression by R848 in M-CSF- versus GM-CSF-derived macrophages after LCMV infection.The Journal of general virology · 2021Article
- Chemokine Signatures of Pathogen-Specific T Cells II: Memory T Cells in Acute and Chronic Infection.Journal of immunology (Baltimore, Md. : 1950) · 2020Article
- A Cell-Based Capture Assay for Rapid Virus Detection.Viruses · 2020Article
- Chronic Lymphocytic Choriomeningitis Infection Causes Susceptibility to Mousepox and Impairs Natural Killer Cell Maturation and Function.Journal of virology · 2020Article
- Loss of Resistance to Mousepox during Chronic Lymphocytic Choriomeningitis Virus Infection Is Associated with Impaired T-Cell Responses and Can Be Rescued by Immunization.Journal of virology · 2020Article
- B cells are the predominant mediators of early systemic viral dissemination during rectal LCMV infection.Mucosal immunology · 2018Article
- Semi-Functional Quantitative Flow Cytometry Assay for Lymphocytic Choriomeningitis Virus Titration.Immune network · 2017Article
- Host genetics play a critical role in controlling CD8 T cell function and lethal immunopathology during chronic viral infection.PLoS pathogens · 2017Article
- M(IL-4) Tissue Macrophages Support Efficient Interferon-Gamma Production in Antigen-Specific CD8Frontiers in immunology · 2017Article
- CXCR5(+) follicular cytotoxic T cells control viral infection in B cell follicles.Nature immunology · 2016Article
- The Transcription Factor AP4 Mediates Resolution of Chronic Viral Infection through Amplification of Germinal Center B Cell Responses.Immunity · 2016Article
- Cutting edge: Expression of FcγRIIB tempers memory CD8 T cell function in vivo.Journal of immunology (Baltimore, Md. : 1950) · 2014Article
- An in vitro model to study immune responses of human peripheral blood mononuclear cells to human respiratory syncytial virus infection.Journal of visualized experiments : JoVE · 2013Article
- T-box transcription factor T-bet, a key player in a unique type of B-cell activation essential for effective viral clearance.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- High efficiency of antiviral CD4(+) killer T cells.PloS one · 2013Article
- Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Lymphocytic choriomeningitis virus (LCMV), a natural murine pathogen, is a member of the Arenavirus family, may cause atypical meningitis in humans, and has been utilized extensively as a model pathogen for the study of virus-induced disease and immune responses. Historically, viral titers have been quantified by a standard plaque assay, but for non-cytopathic viruses including LCMV this requires lengthy incubation, so results cannot be obtained rapidly. Additionally, due to specific technical constraints of the plaque assay including the visual detection format, it has an element of subjectivity along with limited sensitivity. In this study, we describe the development of a FACS-based assay that utilizes detection of LCMV nucleoprotein (NP) expression in infected cells to determine viral titers, and that exhibits several advantages over the standard plaque assay. We show that the LCMV-NP FACS assay is an objective and reproducible detection method that requires smaller sample volumes, exhibits a ∼20-fold increase in sensitivity to and produces results three times faster than the plaque assay. Importantly, when applied to models of acute and chronic LCMV infection, the LCMV-NP FACS assay revealed the presence of infectious virus in samples that were determined to be negative by plaque assay. Therefore, this technique represents an accelerated, enhanced and objective alternative method for detection of infectious LCMV that is amenable to adaptation for other viral infections as well as high throughput diagnostic platforms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.