ArticlePloS one2012
In situ identification of plant-invasive bacteria with MALDI-TOF mass spectrometry.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Review
- Characterization of symbiotic and nitrogen fixing bacteria.AMB Express · 2022Review
- Characterisation of the Antibiotic Profile ofMicroorganisms · 2021Article
- Article
- Surface Micro Discharge-Cold Atmospheric Pressure Plasma Processing of Common House CricketFrontiers in bioengineering and biotechnology · 2021Article
- Development of the Correction Algorithm to Limit the Deformation of Bacterial Colonies Diffraction Patterns Caused by Misalignment and Its Impact on the Bacteria Identification in the Proposed Optical Biosensor.Sensors (Basel, Switzerland) · 2020Article
- Rapid detection of Bacillus ionophore cereulide in food products.Scientific reports · 2019Article
- Integrated multi-channel optical system for bacteria characterization and its potential use for monitoring of environmental bacteria.Biomedical optics express · 2019Article
- Intact Staphylococcus Enterotoxin SEB from Culture Supernatant Detected by MALDI-TOF Mass Spectrometry.Toxins · 2019Article
- Deletion of rRNA Operons ofFrontiers in microbiology · 2019Article
- Cowpea (Agriculture, ecosystems & environment · 2018Article
- Identification of Heterotrophic Zinc Mobilization Processes among Bacterial Strains Isolated from Wheat Rhizosphere (Triticum aestivum L.).Applied and environmental microbiology · 2018Article
- Two Major Clades of Bradyrhizobia Dominate Symbiotic Interactions with Pigeonpea in Fields of Côte d'Ivoire.Frontiers in microbiology · 2016Article
- Biomarker- and similarity coefficient-based approaches to bacterial mixture characterization using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS).Scientific reports · 2015Article
- Advanced mass spectrometry technologies for the study of microbial pathogenesis.Current opinion in microbiology · 2014Article
- Volatiles produced by soil-borne endophytic bacteria increase plant pathogen resistance and affect tritrophic interactions.Plant, cell & environment · 2014Article
- Discrimination of Escherichia coli O157, O26 and O111 from other serovars by MALDI-TOF MS based on the S10-GERMS method.PloS one · 2014Article
- Article
- Identification and Classification of Rhizobia by Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry.Journal of proteomics & bioinformaticsArticle
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rhizobia form a disparate collection of soil bacteria capable of reducing atmospheric nitrogen in symbiosis with legumes. The study of rhizobial populations in nature involves the collection of large numbers of nodules found on roots or stems of legumes, and the subsequent typing of nodule bacteria. To avoid the time-consuming steps of isolating and cultivating nodule bacteria prior to genotyping, a protocol of strain identification based on the comparison of MALDI-TOF MS spectra was established. In this procedure, plant nodules were considered as natural bioreactors that amplify clonal populations of nitrogen-fixing bacteroids. Following a simple isolation procedure, bacteroids were fingerprinted by analysing biomarker cellular proteins of 3 to 13 kDa using Matrix Assisted Laser Desorption/Ionization Time of Flight (MALDI-TOF) mass spectrometry. In total, bacteroids of more than 1,200 nodules collected from roots of three legumes of the Phaseoleae tribe (cowpea, soybean or siratro) were examined. Plants were inoculated with pure cultures of a slow-growing Bradyrhizobium japonicum strain G49, or either of two closely related and fast-growing Sinorhizobium fredii strains NGR234 and USDA257, or with mixed inoculants. In the fully automatic mode, correct identification of bacteroids was obtained for >97% of the nodules, and reached 100% with a minimal manual input in processing of spectra. These results showed that MALDI-TOF MS is a powerful tool for the identification of intracellular bacteria taken directly from plant tissues.
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