ArticlePloS one2012
A proposed taxonomy of anaerobic fungi (class neocallimastigomycetes) suitable for large-scale sequence-based community structure analysis.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 130 citations in OpenAlex.
- Advances in Lignocellulose-Degrading Enzyme Discovery from Anaerobic Rumen Fungi.Microorganisms · 2025Review
- Characterization of fungal communities transmitted from sow to piglet.Microbiology spectrum · 2025Article
- Application of MALDI TOF and DART mass spectrometry as novel tools for classification of anaerobic gut fungi strains.Analytical and bioanalytical chemistry · 2025Article
- Assessing the Impact of the Methane Inhibitors 3-Nitrooxypropanol (3-NOP) and Canola Oil on the Rumen Anaerobic Fungi.Animals : an open access journal from MDPI · 2025Article
- The effect of the forage-to-concentrate ratio of the total mixed ration on ruminal microbiota changes in Alpine dairy goats.Journal of animal science · 2025Article
- Anaerobic gut fungal community in ostriches (ISME communications · 2025Article
- Impact of Seasonal Variation in Pasture on Rumen Microbial Community and Volatile Fatty Acids in Grazing Yaks: Insights from High-Altitude Environments.Microorganisms · 2024Article
- Molecular detection of ruminal micro-flora and micro-fauna in Saudi Arabian camels: Effects of season and region.Saudi journal of biological sciences · 2024Article
- Characterization of the ruminal microbiota in sheep and goats fed different levels of tannin-rich Sericea lespedeza hay.Journal of animal science · 2024Article
- Exploring near-infrared spectroscopy and hyperspectral imaging as novel characterization methods for anaerobic gut fungi.FEMS microbes · 2024Article
- Forage lignocellulose is an important factor in driving the seasonal dynamics of rumen anaerobic fungi in grazing yak and cattle.Microbiology spectrum · 2023Article
- The Effects of Breed and Residual Feed Intake Divergence on the Abundance and Active Population of Rumen Microbiota in Beef Cattle.Animals : an open access journal from MDPI · 2022Article
- Seasonal and geographical differences in the ruminal microbial and chloroplast composition of sika deer (Cervus nippon) in Japan.Scientific reports · 2022Article
- Gut fungal community composition analysis of myostatin mutant cattle prepared by CRISPR/Cas9.Frontiers in veterinary science · 2022Article
- Long-term effects of early-life rumen microbiota modulation on dairy cow production performance and methane emissions.Frontiers in microbiology · 2022Article
- Characterization of fungal microbial diversity in healthy and diarrheal Tibetan piglets.BMC microbiology · 2021Article
- The Effect of a High-Grain Diet on the Rumen Microbiome of Goats with a Special Focus on Anaerobic Fungi.Microorganisms · 2021Article
- Article
- Domesticated equine species and their derived hybrids differ in their fecal microbiota.Animal microbiome · 2020Article
- Characterization of Anaerobic Rumen Fungal Community Composition in Yak, Tibetan Sheep and Small Tail Han Sheep Grazing on the Qinghai-Tibetan Plateau.Animals : an open access journal from MDPI · 2020Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Anaerobic fungi are key players in the breakdown of fibrous plant material in the rumen, but not much is known about the composition and stability of fungal communities in ruminants. We analyzed anaerobic fungi in 53 rumen samples from farmed sheep (4 different flocks), cattle, and deer feeding on a variety of diets. Denaturing gradient gel electrophoresis fingerprinting of the internal transcribed spacer 1 (ITS1) region of the rrn operon revealed a high diversity of anaerobic fungal phylotypes across all samples. Clone libraries of the ITS1 region were constructed from DNA from 11 rumen samples that had distinctly different fungal communities. A total of 417 new sequences were generated to expand the number and diversity of ITS1 sequences available. Major phylogenetic groups of anaerobic fungi in New Zealand ruminants belonged to the genera Piromyces, Neocallimastix, Caecomyces and Orpinomyces. In addition, sequences forming four novel clades were obtained, which may represent so far undetected genera or species of anaerobic fungi. We propose a revised phylogeny and pragmatic taxonomy for anaerobic fungi, which was tested and proved suitable for analysis of datasets stemming from high-throughput next-generation sequencing methods. Comparing our revised taxonomy to the taxonomic assignment of sequences deposited in the GenBank database, we believe that >29% of ITS1 sequences derived from anaerobic fungal isolates or clones are misnamed at the genus level.
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