ArticlePLoS pathogens2012
Functional analysis of NopM, a novel E3 ubiquitin ligase (NEL) domain effector of Rhizobium sp. strain NGR234.
Article in PLoS pathogens, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers, 1 of them a synthesis that pooled it.
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Who cites it
68 citing papers in PubMed, 1 synthesis or guideline pooled it, 128 citations in OpenAlex.
- Root colonization by beneficial rhizobacteria.FEMS microbiology reviews · 2024Pooled it
- E3 ubiquitin ligases in plant defense: mechanisms and prospects for resistance breeding.Science China. Life sciences · 2026Review
- Comparative Genomic Analysis of the Type VI Secretion System in Strains of the Rhizobium leguminosarum Species Complex with Variable Host Specificity.Current microbiology · 2026Article
- Coordination of PTI and ETI in legume-rhizobium mutualism.aBIOTECH · 2026Review
- Ubiquitination as a multi-layer regulatory network in legume-rhizobium symbiosis.Plant cell reports · 2026Review
- Phosphorylation of rhizobial effector NopZ by soybean NORK promotes association with NENA and enhances nodulation.Nature communications · 2026Article
- Rhizobial effector NopM mediates the ubiquitination of the Nod factor receptor NFR5 and promotes rhizobial symbiosis in Lotusjaponicus.Plant communications · 2026Article
- Rhizobial Nod factors modulate reactive oxygen species, jasmonates, and pattern-recognizing receptors to suppress immune response.Plant molecular biology · 2026Article
- Article
- Characterizing host-microbe interactions with bacterial effector proteins using proximity-dependent biotin identification (BioID).Communications biology · 2025Review
- Article
- Plant exudates-driven microbiome recruitment and assembly facilitates plant health management.FEMS microbiology reviews · 2025Review
- The Type III Effector NopM from Bradyrhizobium elkanii USDA61 Induces a Hypersensitive Response in Lotus japonicus Root Nodules.Microbes and environments · 2025Article
- The type III effector NopL interacts with GmREM1a and GmNFR5 to promote symbiosis in soybean.Nature communications · 2024Article
- Unravelling bacterial virulence factors in yeast: From identification to the elucidation of their mechanisms of action.Archives of microbiology · 2024Review
- Defense and senescence interplay in legume nodules.Plant communications · 2024Review
- Mechanisms of rhizosphere plant-microbe interactions: molecular insights into microbial colonization.Frontiers in plant science · 2024Review
- Differential symbiotic compatibilities between rhizobium strains and cultivated and wild soybeans revealed by anatomical and transcriptome analyses.Frontiers in plant science · 2024Article
- NopAA and NopD Signaling Association-Related GeneInternational journal of molecular sciences · 2023Article
- G-type receptor-like kinase AsNIP43 interacts with rhizobia effector nodulation outer protein P and is required for symbiosis.Plant physiology · 2023Article
8 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Type 3 effector proteins secreted via the bacterial type 3 secretion system (T3SS) are not only virulence factors of pathogenic bacteria, but also influence symbiotic interactions between nitrogen-fixing nodule bacteria (rhizobia) and leguminous host plants. In this study, we characterized NopM (nodulation outer protein M) of Rhizobium sp. strain NGR234, which shows sequence similarities with novel E3 ubiquitin ligase (NEL) domain effectors from the human pathogens Shigella flexneri and Salomonella enterica. NopM expressed in Escherichia coli, but not the non-functional mutant protein NopM-C338A, showed E3 ubiquitin ligase activity in vitro. In vivo, NopM, but not inactive NopM-C338A, promoted nodulation of the host plant Lablab purpureus by NGR234. When NopM was expressed in yeast, it inhibited mating pheromone signaling, a mitogen-activated protein (MAP) kinase pathway. When expressed in the plant Nicotiana benthamiana, NopM inhibited one part of the plant's defense response, as shown by a reduced production of reactive oxygen species (ROS) in response to the flagellin peptide flg22, whereas it stimulated another part, namely the induction of defense genes. In summary, our data indicate the potential for NopM as a functional NEL domain E3 ubiquitin ligase. Our findings that NopM dampened the flg22-induced ROS burst in N. benthamiana but promoted defense gene induction are consistent with the concept that pattern-triggered immunity is split in two separate signaling branches, one leading to ROS production and the other to defense gene induction.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.