ArticleThe Journal of experimental medicine2012
DNA polymerase ζ generates tandem mutations in immunoglobulin variable regions.
Article in The Journal of experimental medicine, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 45 citations in OpenAlex.
- Molecular mechanisms of DNA lesion and repair during antibody somatic hypermutation.Science China. Life sciences · 2024Review
- Evolutionary Shortcuts via Multinucleotide Substitutions and Their Impact on Natural Selection Analyses.Molecular biology and evolution · 2023Article
- Characterisation of the spectrum and genetic dependence of collateral mutations induced by translesion DNA synthesis.PLoS genetics · 2022Article
- Genome-wide mutational signatures revealed distinct developmental paths for human B cell lymphomas.The Journal of experimental medicine · 2021Article
- Tandem Substitutions in Somatic Hypermutation.Frontiers in immunology · 2021Article
- Bypass of DNA interstrand crosslinks by a Rev1-DNA polymerase ζ complex.Nucleic acids research · 2020Article
- REV7 is required for processing AID initiated DNA lesions in activated B cells.Nature communications · 2020Article
- What Targets Somatic Hypermutation to the Immunoglobulin Loci?Viral immunology · 2020Review
- Mutating for Good: DNA Damage Responses During Somatic Hypermutation.Frontiers in immunology · 2019Review
- Multinucleotide mutations cause false inferences of lineage-specific positive selection.Nature ecology & evolution · 2018Article
- DNA polymerase η mutational signatures are found in a variety of different types of cancer.Cell cycle (Georgetown, Tex.) · 2018Article
- DNA polymerase ι functions in the generation of tandem mutations during somatic hypermutation of antibody genes.The Journal of experimental medicine · 2016Article
- PrimPol prevents APOBEC/AID family mediated DNA mutagenesis.Nucleic acids research · 2016Article
- Antibody diversification caused by disrupted mismatch repair and promiscuous DNA polymerases.DNA repair · 2016Review
- Evaluation of the Antigen-Experienced B-Cell Receptor Repertoire in Healthy Children and Adults.Frontiers in immunology · 2016Article
- The Polymerase Activity of Mammalian DNA Pol ζ Is Specifically Required for Cell and Embryonic Viability.PLoS genetics · 2016Article
- APE1 is dispensable for S-region cleavage but required for its repair in class switch recombination.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Spt5 accumulation at variable genes distinguishes somatic hypermutation in germinal center B cells from ex vivo-activated cells.The Journal of experimental medicine · 2014Article
- Opinion: uracil DNA glycosylase (UNG) plays distinct and non-canonical roles in somatic hypermutation and class switch recombination.International immunology · 2014Review
- Error-prone polymerase activity causes multinucleotide mutations in humans.Genome research · 2014Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Low-fidelity DNA polymerases introduce nucleotide substitutions in immunoglobulin variable regions during somatic hypermutation. Although DNA polymerase (pol) η is the major low-fidelity polymerase, other DNA polymerases may also contribute. Existing data are contradictory as to whether pol ζ is involved. We reasoned that the presence of pol η may mask the contribution of pol ζ, and therefore we generated mice deficient for pol η and heterozygous for pol ζ. The frequency and spectra of hypermutation was unaltered between Polζ(+/-) Polη(-/-) and Polζ(+/+) Polη(-/-) clones. However, there was a decrease in tandem double-base substitutions in Polζ(+/-) Polη(-/-) cells compared with Polζ(+/+) Polη(-/-) cells, suggesting that pol ζ generates tandem mutations. Contiguous mutations are consistent with the biochemical property of pol ζ to extend a mismatch with a second mutation. The presence of this unique signature implies that pol ζ contributes to mutational synthesis in vivo. Additionally, data on tandem mutations from wild type, Polζ(+/-), Polζ(-/-), Ung(-/-), Msh2(-/-), Msh6(-/-), and Ung(-/-) Msh2(-/-) clones suggest that pol ζ may function in the MSH2-MSH6 pathway.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.