ArticleDigestive diseases and sciences2012
Transforming growth factor beta1 gene variation Leu10Pro affects secretion and function in hepatic cells.
Article in Digestive diseases and sciences, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 7 citations in OpenAlex.
- Association ofInternational journal of molecular sciences · 2026Article
- The role of TGF-β1 C-509T and TGF-β1 T869C polymorphisms in susceptibility to tuberculosis infection in a Sudanese population: a case-control study.BMC infectious diseases · 2025Article
- Polymorphisms in the TGFB1 signal peptide influence human papillomavirus infection and development of cervical lesions.Medical microbiology and immunology · 2019Article
- A TGF-β1 genetic variant at the miRNA187 binding site significantly modifies risk of HPV16-associated oropharyngeal cancer.International journal of cancer · 2018Article
- Association of transforming growth factor-β1 polymorphisms with the risk of diabetes mellitus.International journal of clinical and experimental medicine · 2015Article
- Genetic influences on hand osteoarthritis in Finnish women--a replication study of candidate genes.PloS one · 2014Article
- Changes to signal peptide and the level of transforming growth factor- β1 due to T869C polymorphism of TGF β1 associated with lupus renal fibrosis.SpringerPlus · 2014Article
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundOur previous work revealed transforming growth factor beta1 (TGFβ1) gene polymorphisms are associated with susceptibility to hepatocellular carcinoma and liver cirrhosis. However, no further study of functional substitution in hepatic cells has yet been reported.
aimsThis study was designed to uncover the functional mechanisms of TGFβ1 gene polymorphisms in the pathogenesis of liver diseases.
methodsTwo recombinant TGFβ1 expression plasmids containing TGFβ1 codon 10 Leu/Pro variation were constructed with CMV promoter and transfected into human hepatoma cell lines (HepG2 and SMMU 7721), hepatic stellate cells (LX-2), and immortalized hepatocytes (L02). The secretion capacities of TGFβ1 protein in the transfected cells were determined by ELISA. Apoptosis, proliferative activity, and expression of CD 105, CD83, and CD80 were also measured by use of flow cytometry.
resultsThe ELISA results showed that cells transfected with CMV-Pro10 were more capable of TGFβ1 secretion than those transfected with CMV-Leu10. Functionally, CMV-Pro10 was more apoptosis-protective and induced more proliferation than CMV-Leu10 in transfected hepatic cells. Pro10 up-regulated expression of CD105 and down-regulated expression of CD83.
conclusionsTGFβ1 gene Leu10Pro variation in signal peptide has significant effects on TGFβ1 secretion and functions in hepatic cells.
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