ArticleHistochemistry and cell biology2012
Alcohol induces Golgi fragmentation in differentiated PC12 cells by deregulating Rab1-dependent ER-to-Golgi transport.
Article in Histochemistry and cell biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 26 citations in OpenAlex.
- Intracellular traffic and polarity in brain development.Frontiers in neuroscience · 2023Review
- Alcohol and Prostate Cancer: Time to Draw Conclusions.Biomolecules · 2022Review
- Fragmentation of the Golgi complex of dopaminergic neurons in human substantia nigra: New cytopathological findings in Parkinson's disease.Histology and histopathology · 2021Article
- CREB3L2 Modulates Nerve Growth Factor-Induced Cell Differentiation.Frontiers in molecular neuroscience · 2021Article
- Does Inter-Organellar Proteostasis Impact Yeast Quality and Performance During Beer Fermentation?Frontiers in genetics · 2020Article
- Downregulation of the small GTPase SAR1A: a key event underlying alcohol-induced Golgi fragmentation in hepatocytes.Scientific reports · 2015Article
- Chronic alcohol exposure affects the cell components involved in membrane traffic in neuronal dendrites.Neurotoxicity research · 2015Article
- Golgi fragmentation induced by heat shock or inhibition of heat shock proteins is mediated by non-muscle myosin IIA via its interaction with glycosyltransferases.Cell stress & chaperones · 2014Article
- Reduction in Golgi apparatus dimension in the absence of a residential protein, N-acetylglucosaminyltransferase V.Histochemistry and cell biology · 2014Article
- Chronic alcohol alters dendritic spine development in neurons in primary culture.Neurotoxicity research · 2013Article
- Golgi tubules: their structure, formation and role in intra-Golgi transport.Histochemistry and cell biology · 2013Review
- Neuroprotective activities of catalpol against CaMKII-dependent apoptosis induced by LPS in PC12 cells.British journal of pharmacology · 2013Article
- The Histochemistry and Cell Biology compendium: a review of 2012.Histochemistry and cell biology · 2013Review
- Acetaldehyde and parkinsonism: role of CYP450 2E1.Frontiers in behavioral neuroscience · 2013Article
- Time course of SERCA 2b and calreticulin expression in Purkinje neurons of ethanol-fed rats with behavioral correlates.Alcohol and alcoholism (Oxford, Oxfordshire)Article
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the present study, we analyze the effects of ethanol on the Golgi structure and membrane transport in differentiated PC12 cells, which are used as a model of neurons. Chronic exposure to moderate doses of ethanol induces Golgi fragmentation, a common characteristic of many neurodegenerative diseases. Alcohol impaired the lateral linking of stacks without causing microtubule damage. Extensive immunocytochemical and western blot analyses of representative Golgi proteins showed that few, but important, proteins are significantly affected. Thus, alcohol exposure induced a significant ER-to-Golgi transport delay, the retention of the GTPase Rab1 in the Golgi membranes and the accumulation of tethering factor p115 in the cytosol. These modifications would explain the observed fragmentation. The amount of p115 and the stacking protein GRASP65 increased in alcohol-treated cells, which might be a mechanism to reverse Golgi damage. Importantly, the overexpression of GTP-tagged Rab1 but not of a dominant-negative Rab1 mutant, restored the Golgi morphology, suggesting that this protein is the main target of alcohol. Taken together, our results support the view that alcohol and neurodegenerative diseases such as Parkinson have similar effects on intracellular trafficking and provide new clues on the neuropathology of alcoholism.
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