ArticleMicrobial ecology2012
An improved sequence-aided T-RFLP analysis of bacterial succession during oyster mushroom substrate preparation.
Article in Microbial ecology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 32 citations in OpenAlex.
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- The Effects of Fungal Pathogen Infestation on Soil Microbial Communities forJournal of fungi (Basel, Switzerland) · 2025Article
- Dynamics of soil microbiome throughout the cultivation life cycle of morel (Frontiers in microbiology · 2023Article
- Dynamics of microbial community and enzyme activities during preparation of Agaricus bisporus compost substrate.ISME communications · 2022Article
- Impact of Cultivation Substrate and Microbial Community on Improving Mushroom Productivity: A Review.Biology · 2022Review
- Dynamic succession of microbial compost communities and functions duringFrontiers in microbiology · 2022Article
- Rigid bioplastics shape the microbial communities involved in the treatment of the organic fraction of municipal solid waste.Frontiers in microbiology · 2022Article
- Development of a consortium-based microbial agent beneficial to composting of distilled grain waste for Pleurotus ostreatus cultivation.Biotechnology for biofuels · 2021Article
- Succession and potential role of bacterial communities during Pleurotus ostreatus production.FEMS microbiology ecology · 2021Article
- Holistic assessment of the microbiome dynamics in the substrates used for commercial champignon (Agaricus bisporus) cultivation.Microbial biotechnology · 2020Article
- Analysis of biofilm bacterial communities responsible for carbon removal through a reactor cascade treating wastewater.World journal of microbiology & biotechnology · 2014Article
- Remarkable impact of PAHs and TPHs on the richness and diversity of bacterial species in surface soils exposed to long-term hydrocarbon pollution.World journal of microbiology & biotechnology · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
While oyster mushroom (Pleurotus spp.) is one of the most popular cultivated edible mushrooms, there is scanty information about the microbial community taking part in mushroom substrate production. In this study, an improved sequence-aided terminal restriction fragment length polymorphism (T-RFLP) was used to identify and (semi-)quantify the dominant bacteria of oyster mushroom substrate preparation. The main features of the improved T-RFLP data analysis were the alignment of chromatograms with variable clustering thresholds, the visualization of data matrix with principal component analysis ordination superimposed with cluster analysis, and the search for stage-specific peaks (bacterial taxa) with similarity percentage (analysis of similarity) analysis, followed by identification with clone libraries. By applying this method, the dominance of the following bacterial genera was revealed during oyster mushroom substrate preparation: Pseudomonas and Sphingomonas at startup, Bacillus, Geobacillus, Ureibacillus, Pseudoxanthomonas, and Thermobispora at the end of partial composting, and finally several genera of Actinobacteria, Thermus, Bacillus, Geobacillus, Thermobacillus, and Ureibacillus in the mature substrate. As the proportion of uncultured bacteria increased during the process, it is worth establishing strain collections from partial composting and from mature substrate for searching new species.
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