ArticleGut microbes
Activated fluid transport regulates bacterial-epithelial interactions and significantly shifts the murine colonic microbiome.
Article in Gut microbes. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Resveratrol as an Adjunct Antiviral Agent in Pediatric Viral Infections: A Review on Mechanistic Insights and Gut Microbiota Modulation.International journal of molecular sciences · 2025Review
- Targeting Phosphodiesterase 4 in Gastrointestinal and Liver Diseases: From Isoform-Specific Mechanisms to Precision Therapeutics.Biomedicines · 2025Review
- Contact-dependent, polarized acidification response during neutrophil-epithelial interactions.Journal of leukocyte biology · 2022Article
- From the intestinal mucosal barrier to the enteric neuromuscular compartment: an integrated overview on the morphological changes in Parkinson's disease.European journal of histochemistry : EJH · 2021Review
- Gut-associated cGMP mediates colitis and dysbiosis in a mouse model of an activating mutation in GUCY2C.The Journal of experimental medicine · 2021Article
- The Impact of Gut Microbiota on Post-Stroke Management.Frontiers in cellular and infection microbiology · 2021Article
- Air pollution exposure is associated with the gut microbiome as revealed by shotgun metagenomic sequencing.Environment international · 2020Article
- Cyclic nucleotides, gut physiology and inflammation.The FEBS journal · 2020Review
- Regulation of Ion Transport in the Intestine by Free Fatty Acid Receptor 2 and 3: Possible Involvement of the Diffuse Chemosensory System.International journal of molecular sciences · 2018Review
- Guanylate cyclase C reduces invasion of intestinal epithelial cells by bacterial pathogens.Scientific reports · 2018Article
- Neutrophils as Components of Mucosal Homeostasis.Cellular and molecular gastroenterology and hepatology · 2017Review
- IL-22 Upregulates Epithelial Claudin-2 to Drive Diarrhea and Enteric Pathogen Clearance.Cell host & microbe · 2017Article
- Endurance exercise and gut microbiota: A review.Journal of sport and health science · 2017Review
- Endogenous and exogenous control of gastrointestinal epithelial function: building on the legacy of Bayliss and Starling.The Journal of physiology · 2017Article
- High-fat diet modifies the PPAR-γ pathway leading to disruption of microbial and physiological ecosystem in murine small intestine.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Neutrophils and the inflammatory tissue microenvironment in the mucosa.Immunological reviews · 2016Review
- Biological Activities of Uric Acid in Infection Due to Enteropathogenic and Shiga-Toxigenic Escherichia coli.Infection and immunity · 2016Article
- Ex Vivo Intestinal Sacs to Assess Mucosal Permeability in Models of Gastrointestinal Disease.Journal of visualized experiments : JoVE · 2016Article
- Neutrophils and inflammatory resolution in the mucosa.Seminars in immunology · 2015Review
- Article
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8 authors.
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Abstract
Within the intestinal mucosa, epithelial cells serve multiple functions to partition the lumen from the lamina propria. As part of their natural function, intestinal epithelial cells actively transport electrolytes with passive water movement as a mechanism for mucosal hydration. Here, we hypothesized that electrogenic Cl(-) secretion, and associated mucosal hydration, influences bacterial-epithelial interactions and significantly influences the composition of the intestinal microbiota. An initial screen of different epithelial secretagogues identified lubiprostone as the most potent agonist for which to define these principles. In in vitro studies using cultured T84 cells, lubiprostone decreased E. coli translocation in a concentration-dependent manner (p < 0.001) and decreased S. typhimurium internalization and translocation by as much as 71 ± 6% (p < 0.01). Such decreases in bacterial translocation were abolished by inhibition of electrogenic Cl(-) secretion and water transport using the Na/K/Cl(-) antagonist bumetanide (p < 0.01). Extensions of these findings to microbiome analysis in vivo revealed that lubiprostone delivered orally to mice fundamentally shifted the intestinal microbiota, with notable changes within the Firmicutes and Bacteroidetes phyla of resident colonic bacteria. Such findings document a previously unappreciated role for epithelial Cl(-) secretion and water transport in influencing bacterial-epithelial interactions and suggest that active mucosal hydration functions as a primitive innate epithelial defense mechanism.
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